Transcriptome analysis of Nicotiana benthamiana infected by Tobacco curly shoot virus.
Transcriptome analysis of Nicotiana benthamiana infected by Tobacco curly shoot virus.
复制标题
烟草卷枝病毒感染本塞姆氏烟草的转录组分析
DOI：
10.1186/s12985-018-1044-1
复制
发表时间：
2018-09-03
期刊：
影响因子：
4.8
通讯作者：
Qing L
中科院分区：
文献类型：
--
作者：
Li K;Wu G;Li M;Ma M;Du J;Sun M;Sun X;Qing L
烟草曲茎病毒（Tobacco curly shoot virus，TbCSV）是一种与β卫星（Tobacco curly shoot betasatellite，TbCSB）共生的单分型菜豆花叶病毒，在我国番茄和烟草上引起严重的曲叶病。有趣的是，TbCSV诱导了本氏烟草严重的向上卷叶，但在TbCSB的存在下，症状变为向下卷叶。然而，病毒致病性、宿主防御、病毒-β卫星相互作用和病毒-宿主相互作用之间的相互作用机制仍不清楚。本研究利用RNA-seq技术分析了N.分别用TbCSV（Y35 A）和TbCSV + TbCSB（Y35 AB）侵染本氏烟（benthamiana）。通过映射到N。在本塞姆氏菌参考基因组中，鉴定了59，814个单基因。转录组分析显示，在Y35 AB与CK（对照检查）和Y35 A与CK中分别鉴定了总共4081个和3196个DEG。进行GO和KEGG分析以分类DEG。前15个GO术语中有10个在Y35 AB vs CK和Y35 A vs CK两个DEG中都得到了富集，这些富集的GO术语主要分为生物过程、细胞组分和分子功能三类。KEGG途径分析表明，Y35 AB和Y35 A分别鉴定出118条和111条KEGG途径，其中9条和6条KEGG途径明显富集。Y35 AB与CK的3条主要途径涉及代谢途径、碳代谢和光合作用，而Y35 A与CK的3条主要途径涉及核糖体、乙醛酸和二羧酸代谢以及DNA复制。我们观察到8个PR基因在Y35 A和Y35 AB处理中显著上调，44个LRR-RLK基因在Y35 A和Y35 AB处理中显著差异表达。另外，在油菜素内酯（BR）和茉莉酸（JA）的生物合成和信号转导途径中，分别有7个和13个基因发生了显著变化。这些结果将有助于进一步阐明寄主植物对病毒感染的反应。
Tobacco curly shoot virus (TbCSV) is a monopartite begomovirus associated with betasatellite (Tobacco curly shoot betasatellite, TbCSB), which causes serious leaf curl disease on tomato and tobacco in China. It is interesting that TbCSV induced severe upward leaf curling in Nicotiana benthamiana, but in the presence of TbCSB, symptoms changed to be downward leaf curling. However, the mechanism of interactions between viral pathogenicity, host defense, viral-betasatellite interactions and virus-host interactions remains unclear. In this study, RNA-seq was used to analyze differentially expressed genes (DEGs) in N. benthamiana plants infected by TbCSV (Y35A) and TbCSV together with TbCSB (Y35AB) respectively. Through mapping to N. benthamiana reference genome, 59,814 unigenes were identified. Transcriptome analysis revealed that a total of 4081 and 3196 DEGs were identified in Y35AB vs CK (control check) and Y35A vs CK, respectively. Both GO and KEGG analyses were conducted to classify the DEGs. Ten of the top 15 GO terms were enriched in both DEGs of Y35AB vs CK and Y35A vs CK, and these enriched GO terms mainly classified into three categories including biological process, cellular component and molecular function. KEGG pathway analysis indicated that 118 and 111 pathways were identified in Y35AB vs CK and Y35A vs CK, respectively, of which nine and six pathways were significantly enriched. Three major pathways in Y35AB vs CK involved in metabolic pathways, carbon metabolism and photosynthesis, while those in Y35A vs CK were related to Ribosome, Glyoxylate and dicarboxylate metabolism and DNA replication. We observed that 8 PR genes were significantly up-regulated and 44 LRR-RLK genes were significantly differentially expressed in Y35A treatment or in Y35AB treatment. In addition, 7 and 13 genes were identified to be significantly changed in biosynthesis and signal transduction pathway of brassinosteroid (BR) and jasmonic acid (JA) respectively. These results presented here would be particularly useful to further elucidate the response of the host plant against virus infection.
登录
查看更多内容
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
5.4
作者：
Cui, XF;Tao, XR;Zhou, XP
通讯作者：
Zhou, XP
影响因子：
11.6
作者：
Albrecht, C;Russinova, E;de Vries, S
通讯作者：
de Vries, S
影响因子：
10.5
作者：
Fontes, EPB;Santos, AA;Chory, J
通讯作者：
Chory, J
影响因子：
3.1
作者：
Abarshi, M. M.;Mohammed, I. U.;Maruthi, M. N.
通讯作者：
Maruthi, M. N.