Daphnoretin induces cell cycle arrest and apoptosis in human osteosarcoma (HOS) cells.
Daphnoretin induces cell cycle arrest and apoptosis in human osteosarcoma (HOS) cells.
复制标题
致富诺列汀在人骨肉瘤（HOS）细胞中诱导细胞周期停滞和凋亡。
DOI：
10.3390/molecules17010598
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发表时间：
2012-01-09
期刊：
影响因子：
--
通讯作者：
He J
中科院分区：
文献类型：
--
作者：
Gu S;He J
本研究观察瑞香素对人骨肉瘤(HOS)细胞的抗增殖、细胞周期停滞和细胞凋亡的作用。用四甲基偶氮唑蓝(3-(4，5-dimethylthiazol-2-yl)-2，5-diphenyltetrazolium)比色法测定细胞增殖活性。瑞香素作用72 h的IC50值为3.89μM。凋亡小体的出现和Annexin V-FITC/PI细胞凋亡检测试剂盒证实了细胞的凋亡诱导。流式细胞仪分析显示瑞香素使细胞周期停滞于G2/M期。Western-blotting分析显示，细胞周期停滞于G2/M期时伴随着cdc2、Cyclin A和Cyclin B1的下调。瑞香素还能抑制Bcl2的表达，诱导Bax的表达使线粒体膜解体，引起细胞色素c的释放。线粒体细胞色素c的释放与caspase-9和caspase-3级联激活有关。我们的结果表明瑞香素通过连续阻断细胞于G2/M期并激活caspase-3通路而导致HOS细胞死亡。
In this study antiproliferation, cell cycle arrest and apoptosis induced by daphnoretin in human osteosarcoma (HOS) cells were investigated. Antiproliferative activity was measured with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The IC50 value of daphnoretin was 3.89 μM after 72 h treatment. Induction of apoptosis was evidenced by apoptotic body appearance and Annexin V-FITC/PI apoptosis detection kit. Flow cytometric analysis indicated daphnoretin arrested the cell cycle in the G2/M phase. Western-blot assay showed that the G2/M phase arrest was accompanied by down-regulation of cdc2, cyclin A and cyclin B1. Moreover, daphnoretin inhibited Bcl-2 expression and induced Bax expression to desintegrate the outer mitochondrial membrane and causing cytochrome c release. Mitochondrial cytochrome c release was associated with the activation of caspase-9 and caspase-3 cascade. Our results demonstrated that daphnoretin caused death of HOS cells by blocking cells successively in G2/M phases and activating the caspase-3 pathway.
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影响因子：
1.7
作者：
Jiang, ZH;Tanaka, T;Zhou, RH
通讯作者：
Zhou, RH
影响因子：
3.5
作者：
Debatin, KM
通讯作者：
Debatin, KM
影响因子：
5.8
作者：
Jeong, HW;Han, DC;Kwon, BM
通讯作者：
Kwon, BM
影响因子：
2.8
作者：
Fujimori, Jun;Matsuo, Toshihiro;Ochi, Mitsuo
通讯作者：
Ochi, Mitsuo
影响因子：
6.2
作者：
Mirabello, Lisa;Troisi, Rebecca J.;Savage, Sharon A.
通讯作者：
Savage, Sharon A.