Evaluating the diagnostic and prognostic value of circulating cathepsin S in gastric cancer.
Evaluating the diagnostic and prognostic value of circulating cathepsin S in gastric cancer.
复制标题
评估循环组织蛋白酶 S 在胃癌中的诊断和预后价值。
DOI：
10.18632/oncotarget.8582
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发表时间：
2016-05-10
期刊：
影响因子：
--
通讯作者：
Zhang G
中科院分区：
文献类型：
--
作者：
Liu WL;Liu D;Cheng K;Liu YJ;Xing S;Chi PD;Liu XH;Xue N;Lai YZ;Guo L;Zhang G
为探讨血清组织蛋白酶S(Cat S)能否作为胃癌诊断和预后的生物标志物，采用酶联免疫吸附试验(EL ISA)检测了4 96例健康人和胃良性疾病、胃癌、食道癌、肝癌、结直肠癌、鼻咽癌和肺癌患者血清中的Cat S。肿瘤患者血清Cat S水平明显升高，其中以GC患者升高最为明显。定量逆转录聚合酶链式反应、Western blotting和免疫组织化学染色显示，猫科动物S在胃癌细胞系和组织中均有过表达。血清CAT S对正常人胃癌的诊断价值为0.803，敏感性为60.7%，特异性为90.0%。此外，血清Cat S水平与胃癌的肿瘤体积、淋巴结状况、转移状况和分期有关。此外，血清Cat S水平高的患者总体生存较差。单因素分析显示，Cat S的表达是影响预后的因素。Cat S基因的敲除显著抑制了GC细胞的迁移和侵袭。本研究提示血清Cat S可作为胃癌诊断及预后判断的一种潜在的生物标志物。
To evaluate whether serum Cathepsin S (Cat S) could serve as a biomarker for the diagnosis and prognosis of gastric cancer (GC), Enzyme-linked immuno sorbent assay (ELISA) was used to detect serum Cat S in 496 participants including healthy controls and patients with benign gastric diseases, gastric cancer, esophageal cancer, liver cancer, colorectal cancer, nasopharyngeal cancer and lung cancer. The levels of serum Cat S were significantly increased in cancer patients, especially in GC patients. The qRT-PCR, Western blotting, and immunohistochemical staining revealed the overexpression of Cat S in GC cell lines and tissues. The diagnostic value of serum Cat S for GC patients from controls resulted in an AUC of 0.803 with a sensitivity of 60.7% and a specificity of 90.0%. Moreover, the levels of serum Cat S were associated with GC tumor volume, lymphoid nodal status, metastasis status, and stages. Moreover, the patients with high levels of serum Cat S had a poorer overall survival. Univariate analysis revealed Cat S expression was a prognostic factor. The knockdown of Cat S significantly suppressed the migration and invasion of GC cells. This study suggested serum Cat S may be a potential biomarker for the diagnosis and prognosis of GC.
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影响因子：
8.8
作者：
Gormley, J. A.;Hegarty, S. M.;O'Grady, A.;Stevenson, M. R.;Burden, R. E.;Barrett, H. L.;Scott, C. J.;Johnston, J. A.;Wilson, R. H.;Kay, E. W.;Johnston, P. G.;Olwill, S. A.
通讯作者：
Olwill, S. A.
影响因子：
6
作者：
Flannery, T;Gibson, D;Johnston, PG
通讯作者：
Johnston, PG
影响因子：
1.1
作者：
Flannery, T.;Mcconnell, R. S.;Johnston, P. G.
通讯作者：
Johnston, P. G.
影响因子：
1.5
作者：
Czyzewska, Jolanta;Guzinska-Ustymowicz, Katarzyna;Bandurski, Roman
通讯作者：
Bandurski, Roman
影响因子：
7.2
作者：
Huang, C. -C.;Lee, T. -J.;Tsai, C. -N.
通讯作者：
Tsai, C. -N.