Contribution of the drought tolerance-related stress-responsive NAC1 transcription factor to resistance of barley to Ramularia leaf spot.
Contribution of the drought tolerance-related stress-responsive NAC1 transcription factor to resistance of barley to Ramularia leaf spot.
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DOI：
10.1111/mpp.12173
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发表时间：
2015-02
影响因子：
4.9
通讯作者：
Brown JK
中科院分区：
文献类型：
--
作者：
McGrann GR;Steed A;Burt C;Goddard R;Lachaux C;Bansal A;Corbitt M;Gorniak K;Nicholson P;Brown JK
NAC蛋白是植物转录因子，参与对非生物和生物胁迫的耐受性以及许多发育过程。胁迫响应性NAC1（SNAC1）转录因子参与大麦和水稻的耐旱性，但此前未表明其在抗病性方面有作用。在大麦品种Golden Promise中HvSNAC1的转基因过表达降低了由真菌Ramularia collo - cygni引起的叶斑病（RLS）的严重程度，但对由禾谷镰刀菌、雪霉叶枯病菌（眼斑病）、禾本科布氏白粉菌（白粉病）或稻瘟病菌引起的病害症状没有影响。在感染叶片衰老时，在RLS易感品种Golden Promise中，HvSNAC1转录本在R. collo - cygni症状发展的后期被微弱诱导。对控制HvSNAC1介导的对RLS抗性的潜在机制进行了研究。基因表达分析显示，与Golden Promise品种相比，两个过表达株系中抗氧化转录本的组成型水平没有差异，在气孔导度或对活性氧诱导的细胞死亡的敏感性方面也没有观察到任何差异。HvSNAC1的过表达延迟了黑暗诱导的叶片衰老。据推测，由HvSNAC1控制的参与非生物胁迫耐受性且抑制衰老的机制也赋予了对R. collo - cygni的抗性并抑制了RLS症状。这为大麦中非生物胁迫和衰老与RLS发展之间的关联提供了进一步的证据。
NAC proteins are plant transcription factors that are involved in tolerance to abiotic and biotic stresses, as well as in many developmental processes. Stress‐responsive NAC1 (SNAC1) transcription factor is involved in drought tolerance in barley and rice, but has not been shown previously to have a role in disease resistance. Transgenic over‐expression of HvSNAC1 in barley cv. Golden Promise reduced the severity of Ramularia leaf spot (RLS), caused by the fungus Ramularia collo‐cygni, but had no effect on disease symptoms caused by Fusarium culmorum, Oculimacula yallundae (eyespot), Blumeria graminis f. sp. hordei (powdery mildew) or Magnaporthe oryzae (blast). The HvSNAC1 transcript was weakly induced in the RLS‐susceptible cv. Golden Promise during the latter stages of R. collo‐cygni symptom development when infected leaves were senescing. Potential mechanisms controlling HvSNAC1‐mediated resistance to RLS were investigated. Gene expression analysis revealed no difference in the constitutive levels of antioxidant transcripts in either of the over‐expression lines compared with cv. Golden Promise, nor was any difference in stomatal conductance or sensitivity to reactive oxygen species‐induced cell death observed. Over‐expression of HvSNAC1 delayed dark‐induced leaf senescence. It is proposed that mechanisms controlled by HvSNAC1 that are involved in tolerance to abiotic stress and that inhibit senescence also confer resistance to R. collo‐cygni and suppress RLS symptoms. This provides further evidence for an association between abiotic stress and senescence in barley and the development of RLS.
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影响因子：
3.5
作者：
BOYD, LA;SMITH, PH;BROWN, JKM
通讯作者：
BROWN, JKM
影响因子：
3.1
作者：
Al Abdallat, A. M.;Ayad, J. Y.;Harwood, W. A.
通讯作者：
Harwood, W. A.
影响因子：
7.4
作者：
Burton, RA;Shirley, NJ;Fincher, GB
通讯作者：
Fincher, GB
影响因子：
7.4
作者：
Gan, SS;Amasino, RM
通讯作者：
Amasino, RM
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影响因子：
4.4
作者：
Narsai R;Wang C;Chen J;Wu J;Shou H;Whelan J
通讯作者：
Whelan J