Morphology-Variable Aggregates Prepared from Cholesterol-Containing Amphiphilic Glycopolymers: Their Protein Recognition/Adsorption and Drug Delivery Applications.
Morphology-Variable Aggregates Prepared from Cholesterol-Containing Amphiphilic Glycopolymers: Their Protein Recognition/Adsorption and Drug Delivery Applications.
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由含胆固醇两亲性糖聚合物制备的形态可变聚集体：其蛋白质识别/吸附和药物输送应用
DOI：
10.3390/nano8030136
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发表时间：
2018-02-28
期刊：
影响因子：
--
通讯作者：
Sheng R
中科院分区：
文献类型：
--
作者：
Wang Z;Luo T;Cao A;Sun J;Jia L;Sheng R
本研究通过可逆加成-断裂链转移（RAFT）聚合和脱保护反应合成了一系列胆固醇/半乳糖接枝的聚（6-O-甲基丙烯酰基-d-吡喃半乳糖）-b-聚（6-胆固醇氧基己基甲基丙烯酸酯）（PMAgala-b-PMAChols）嵌段共聚物。通过透射电子显微镜（TEM）和动态激光光散射（DLS）研究发现，糖基共聚物可以自组装成具有不同形态的聚集体，其形态取决于胆固醇/半乳糖嵌段的重量比。此外，凝集素（蓖麻凝集素II，RCA 120）识别和牛血清白蛋白（BSA）吸附的PMAgala-b-PMAChol聚集体进行了评价。在体外进一步检查PMAgala-b-PMAChol/多柔比星（DOX）复合物聚集体的SK-Hep-1肿瘤细胞抑制性质。结果表明，具有不同形态的PMAgala-b-PMAChol聚集体与RCA 120和BSA表现出不同的相互作用/识别特征。球形聚集体（d = 92 nm）具有最高的RCA 120识别能力和最低的BSA蛋白吸附。此外，DOX负载的球形复合物聚集体表现出比纳米纤维复合物聚集体更好的肿瘤细胞抑制性能。由两亲性糖基共聚物衍生的形态可变的聚集体可用作具有生物分子识别和药物递送特征的多功能生物材料。
In this study, a series of diblock glycopolymers, poly(6-O-methacryloyl-d-galactopyranose)-b-poly(6-cholesteryloxyhexyl methacrylate) (PMAgala-b-PMAChols), with cholesterol/galactose grafts were prepared through a sequential reversible addition-fragmentation chain transfer (RAFT) polymerization and deprotection process. The glycopolymers could self-assemble into aggregates with various morphologies depending on cholesterol/galactose-containing block weight ratios, as determined by transmission electronic microscopy (TEM) and dynamic laser light scattering (DLS). In addition, the lectin (Ricinus communis agglutinin II, RCA120) recognition and bovine serum albumin (BSA) adsorption of the PMAgala-b-PMAChol aggregates were evaluated. The SK-Hep-1 tumor cell inhibition properties of the PMAgala-b-PMAChol/doxorubicin (DOX) complex aggregates were further examined in vitro. Results indicate that the PMAgala-b-PMAChol aggregates with various morphologies showed different interaction/recognition features with RCA120 and BSA. Spherical aggregates (d ≈ 92 nm) possessed the highest RCA120 recognition ability and lowest BSA protein adsorption. In addition, the DOX-loaded spherical complex aggregates exhibited a better tumor cell inhibition property than those of nanofibrous complex aggregates. The morphology-variable aggregates derived from the amphiphilic glycopolymers may serve as multifunctional biomaterials with biomolecular recognition and drug delivery features.
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影响因子：
29.4
作者：
Jiang, Xuan;Qu, Wei;Pan, Deng;Ren, Yong;Williford, John-Michael;Cui, Honggang;Luijten, Erik;Mao, Hai-Quan
通讯作者：
Mao, Hai-Quan
影响因子：
15
作者：
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通讯作者：
Heise, Andreas
影响因子：
16.1
作者：
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通讯作者：
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影响因子：
3.4
作者：
Jia, Lin;Cao, Amin;Li, Min-Hui
通讯作者：
Li, Min-Hui
影响因子：
14
作者：
Lee, Ashlynn L. Z.;Venkataraman, Shrinivas;Yang, Yi Yan
通讯作者：
Yang, Yi Yan