Insights from theory and experiments on slip flow in chromatography.
Insights from theory and experiments on slip flow in chromatography.
复制标题
DOI：
10.1002/jssc.201300462
复制
发表时间：
2013-06
影响因子：
3.1
通讯作者：
Wirth, Mary J.
中科院分区：
文献类型：
--
作者：
Wu, Zhen;Rogers, Benjamin J.;Wei, Bingchuan;Wirth, Mary J.
滑移流已经成为反相液相色谱中感兴趣的主题，因为它提供了流动增强，促进了亚微米颗粒的使用，提供了分离效率的大幅提高。此外，滑移流通过减小移动的相中的速度分布而提供了效率的额外改进。本文用色谱相关术语描述了开口管中的滑移流现象。最近在这本杂志上的一篇论文进行了讨论，因为它提供了第一个理论研究的滑移流在填充床，在这种情况下，为面心立方几何。该理论论文揭示了填充床的存在引入了流体速度的不均匀性，这在开口管中是不存在的，减少了滑移流对效率的额外提高。最近的论文还表明，还有另一个提高效率的因素，即从停滞流动区域排除蛋白质。后者支持最近发表的数据限制蛋白质扩散面心立方二氧化硅胶体晶体。通过使用亚微米颗粒能够实现极低的板高度，并且当分析物尺寸为粒径的1%或更大时，进一步的改进似乎是可能的。
Slip flow has become a topic of interest in reversed-phase liquid chromatography because it gives a flow enhancement that facilitates the use of submicrometer particles, providing a large improvement in separation efficiency. Moreover, slip flow provides an additional improvement in efficiency by reducing the velocity distribution in the mobile phase. The phenomenon of slip flow in open tubes is described in chromatographically relative terms. A recent paper in this journal is discussed, as it provides the first theoretical study of slip flow in packed beds, in this case for face-centered cubic geometry. The theory paper reveals that the presence of the packed bed introduces a heterogeneity in fluid velocities that is absent in open tubes, reducing the additional improvement in efficiency from slip flow. The recent paper also suggests that there is yet another factor improving efficiency, which is size-exclusion of proteins from regions of stagnant flow. The latter is supported by recently published data on restricted protein diffusion in face-centered cubic silica colloidal crystals. Extremely low plate heights are enabled by use of submicrometer particles, and further improvement appears to be possible when the analyte size is on the order of 1% of the particle diameter or larger.
登录
查看更多内容
影响因子：
--
作者：
Washburn, EW
通讯作者：
Washburn, EW
影响因子：
56.9
作者：
Holt, JK;Park, HG;Bakajin, O
通讯作者：
Bakajin, O
影响因子：
2.9
作者：
Rogers, Benjamin J.;Wirth, Mary J.
通讯作者：
Wirth, Mary J.
有序颗粒堆积和滑流对色谱性能影响的数值研究。
影响因子：
3.1
作者：
Yan, Xiaohong;Wang, Qiuwang
通讯作者：
Wang, Qiuwang
影响因子：
4.1
作者：
Cabooter, Deirdre;Billen, Jeroen;Desmet, Gert
通讯作者：
Desmet, Gert