Cell division cycle 2 participates in eyestalk ablation-induced ovarian maturation of Procambarus clarkii
Cell division cycle 2 participates in eyestalk ablation-induced ovarian maturation of Procambarus clarkii
复制标题
细胞分裂周期2参与克氏原螯虾眼柄消融诱导的卵巢成熟
DOI：
10.1016/j.aquaculture.2016.10.009
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发表时间：
2017-02
期刊：
影响因子：
4.5
通讯作者：
Xiang-Ru Liao
中科院分区：
文献类型：
--
作者：
Zheng-Bing Guan;Hai-Xi Yi;Hong Zhao;Yan Shui;Yu-Jie Cai;Xiang-Ru Liao
眼柄消融通常用于促进圈养经济甲壳类动物卵巢的发育和成熟。了解克氏原螯虾眼柄切除的分子机制对于寻找触发该物种卵巢成熟而不产生不良影响的替代措施至关重要。细胞分裂周期2 （Cdc2）是成熟促进因子的催化亚基，成熟促进因子是诱导卵母细胞减数分裂成熟的核心因子。本研究分析了Cdc2基因表达模式和黄体酮水平。注射或不注射合成pcGnRH对克拉氏卵巢眼柄消融诱导成熟过程的影响。利用RNA干扰技术阐明Cdc2在眼柄消融机制中的作用。结合本研究和前期研究结果，提示卵巢中可能存在孕激素介导的卵母细胞成熟通路。此外，该通路可能在眼柄消融诱导的卵巢成熟过程中发挥重要作用。clarkii。本研究结果支持利用Cdc2功能控制克氏原螯虾卵母细胞成熟。
Eyestalk ablation is commonly used to promote ovarian development and maturation in captive economic crustaceans. Understanding the molecular mechanism of eyestalk ablation inProcambarus clarkiiis essential in finding alternative measures to trigger ovarian maturation on this species without undesirable effects. The cell division cycle 2 (Cdc2) is a catalytic subunit of the maturation-promoting factor, which is a central factor for inducing oocyte meiotic maturation. This study analyzed the Cdc2 gene expression patterns and the progesterone level in theP. clarkiiovary during its eyestalk ablation-induced maturation course with or without synthetic pcGnRH injection. The RNA interference technique was used to elucidate the role of Cdc2 in the eyestalk ablation mechanism. A combination of the present and previous results revealed that the progesterone-mediated oocyte maturation pathway could exist in the ovary. Furthermore, this pathway could play a crucial role in the eyestalk ablation-induced ovarian maturation inP. clarkii.Statement of relevanceThe results of this study enable the utilization of the Cdc2 function to control oocyte maturation in Procambarus clarkii.
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影响因子：
--
作者：
G. Leilei
通讯作者：
G. Leilei
影响因子：
3.5
作者：
Brothers, Shaun P.;Janovick, Jo Ann;Conn, P. Michael
通讯作者：
Conn, P. Michael
影响因子：
6.8
作者：
R. Sarojini;R. Nagabhushanam;M. Fingerman
通讯作者：
R. Sarojini;R. Nagabhushanam;M. Fingerman
DOI：
10.1016/s1095-6433(01)00385-3
发表时间：
2001-09
期刊：
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
影响因子：
--
作者：
S. Warrier;R. Tirumalai;T. Subramoniam
通讯作者：
S. Warrier;R. Tirumalai;T. Subramoniam
克氏原螯虾卵巢中新型促性腺激素释放激素（GnRH）的一级结构
影响因子：
4.5
作者：
Guan, Zheng-Bing;Shui, Yan;Zhou, Xin
通讯作者：
Zhou, Xin