Identification of key candidate genes and miRNA‑mRNA target pairs in chronic lymphocytic leukemia by integrated bioinformatics analysis.
Identification of key candidate genes and miRNA‑mRNA target pairs in chronic lymphocytic leukemia by integrated bioinformatics analysis.
复制标题
通过综合生物信息学分析鉴定慢性淋巴细胞白血病的关键候选基因和 miRNA-mRNA 靶点对。
DOI：
10.3892/mmr.2018.9636
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发表时间：
2019-01
影响因子：
3.4
通讯作者：
Sun C
中科院分区：
文献类型：
--
作者：
Gao C;Zhou C;Zhuang J;Liu L;Wei J;Liu C;Li H;Sun C
慢性淋巴细胞白血病（CLL）是一种恶性克隆性增殖性B细胞疾病。细胞凋亡抑制和细胞周期阻滞是本病的主要病理原因，但其分子机制尚需进一步研究。本研究的目的是寻找CLL早期诊断和治疗的生物标志物，并探讨CLL进展的分子机制。通过分析基因芯片GSE 22529、GSE 39411和GSE 62137，共鉴定出488个CLL差异表达基因（DEG）和32个差异表达microRNA（miRNA; DEM）。DEG的功能和途径富集分析表明，DEG主要参与转录调控和多种信号通路，如核因子-κB和丝裂原活化蛋白激酶信号通路。此外，使用Cytoscape软件来可视化这些DEG的蛋白质-蛋白质相互作用，以鉴定中心基因，其可用作CLL的早期诊断和治疗的生物标志物。Cytoscape软件还用于分析DEM和DEG的预测靶mRNA之间的关联，并增加有关与CLL进展相关的miRNA-mRNA调控网络的知识。总之，本研究通过鉴定差异表达的枢纽基因、miRNA-mRNA靶对和分子通路，为我们进一步理解CLL的发病机制提供了生物信息学基础。此外，中枢基因可用作诊断CLL的新型生物标志物并指导CLL药物组合的选择。
Chronic lymphocytic leukemia (CLL) is a malignant clonal proliferative disorder of B cells. Inhibition of cell apoptosis and cell cycle arrest are the main pathological causes of this disease, but its molecular mechanism requires further investigation. The purpose of the present study was to identify biomarkers for the early diagnosis and treatment of CLL, and to explore the molecular mechanisms of CLL progression. A total of 488 differentially expressed genes (DEGs) and 32 differentially expressed microRNAs (miRNAs; DEMs) for CLL were identified by analyzing the gene chips GSE22529, GSE39411 and GSE62137. Functional and pathway enrichment analyses of DEGs demonstrated that DEGs were mainly involved in transcriptional dysregulation and multiple signaling pathways, such as the nuclear factor-κB and mitogen-activated protein kinase signaling pathways. In addition, Cytoscape software was used to visualize the protein-protein interactions of these DEGs in order to identify hub genes, which could be used as biomarkers for the early diagnosis and treatment of CLL. Cytoscape software was also used to analyze the association between the predicted target mRNAs of DEMs and DEGs and increase knowledge about the miRNA-mRNA regulatory network associated with the progression of CLL. Taken together, the present study provided a bioinformatics basis for advancing our understanding of the pathogenesis of CLL by identifying differentially expressed hub genes, miRNA-mRNA target pairs and molecular pathways. In addition, hub genes may be used as novel biomarkers for the diagnosis of CLL and to guide the selection of CLL drug combinations.
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影响因子：
2.6
作者：
Kamaraj, Balu;Gopalakrishnan, Chandrasekhar;Purohit, Rituraj
通讯作者：
Purohit, Rituraj
SMAD介导的microRNA生物合成调节。
影响因子：
3.5
作者：
Blahna MT;Hata A
通讯作者：
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影响因子：
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作者：
Bhaumik P;Gopalakrishnan C;Kamaraj B;Purohit R
通讯作者：
Purohit R
影响因子：
29.7
作者：
Kaser A;Zeissig S;Blumberg RS
通讯作者：
Blumberg RS
影响因子：
4.5
作者：
He X;Zhang J
通讯作者：
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