The quantitative significance of Syntrophaceae and syntrophic partnerships in methanogenic degradation of crude oil alkanes.
The quantitative significance of Syntrophaceae and syntrophic partnerships in methanogenic degradation of crude oil alkanes.
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DOI：
10.1111/j.1462-2920.2011.02570.x
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发表时间：
2011-11
影响因子：
5.1
通讯作者：
Head IM
中科院分区：
文献类型：
--
作者：
Gray ND;Sherry A;Grant RJ;Rowan AK;Hubert CR;Callbeck CM;Aitken CM;Jones DM;Adams JJ;Larter SR;Head IM
16S rRNA基因的文库中克隆的产甲烷石油降解微宇宙修订与北海原油和接种河口沉积物表明，属Smithella（三角洲变形菌，Syntrophaceace）和Marinaproteobacteria。（γ变形菌）的细菌在降解过程中富集。使用qPCR和烷烃（降解的主要碳氢化合物）的定量数据确定生长产量和倍增时间（Smithella和Marinidae均为36天）。Smithella sp.的生长产率[0.020 g（细胞-C）/g（烷烃-C）]，假设它利用了去除的所有烷烃，与产甲烷聚生体中降解烃和其他有机化合物的细菌的产率一致。在450 d以上的培养过程中，Smithella的优势生长和指数生长与烷烃的去除和甲烷的指数积累相一致。这种增长与Smithella在近地表缺氧烃降解系统中的发生以及它们在这种系统中与产甲烷菌的互养伙伴关系中将原油烷烃完全氧化成乙酸酯和/或氢是一致的。计算的海蛞蝓的生长产量，假设它在烷烃上生长，则为[0.0005 g（细胞-C）/g（烷烃-C）]，表明它在烷烃降解中起次要作用。优势产甲烷菌为氢氧化菌（Methanocalculus spp.）来自甲烷微生物目）。富氢氧化产甲烷菌相对于乙酸裂解产甲烷菌是一致的产甲烷原油降解富集培养物中测得的互养乙酸氧化。甲烷微生物目的qPCR表明与Smithella合作的甲烷生产和生长的测量速率一致的生长特征。
Libraries of 16S rRNA genes cloned from methanogenic oil degrading microcosms amended with North Sea crude oil and inoculated with estuarine sediment indicated that bacteria from the genera Smithella (Deltaproteobacteria, Syntrophaceace) and Marinobacter sp. (Gammaproteobacteria) were enriched during degradation. Growth yields and doubling times (36 days for both Smithella and Marinobacter) were determined using qPCR and quantitative data on alkanes, which were the predominant hydrocarbons degraded. The growth yield of the Smithella sp. [0.020 g(cell-C)/g(alkane-C)], assuming it utilized all alkanes removed was consistent with yields of bacteria that degrade hydrocarbons and other organic compounds in methanogenic consortia. Over 450 days of incubation predominance and exponential growth of Smithella was coincident with alkane removal and exponential accumulation of methane. This growth is consistent with Smithella's occurrence in near surface anoxic hydrocarbon degrading systems and their complete oxidation of crude oil alkanes to acetate and/or hydrogen in syntrophic partnership with methanogens in such systems. The calculated growth yield of the Marinobacter sp., assuming it grew on alkanes, was [0.0005 g(cell-C)/g(alkane-C)] suggesting that it played a minor role in alkane degradation. The dominant methanogens were hydrogenotrophs (Methanocalculus spp. from the Methanomicrobiales). Enrichment of hydrogen-oxidizing methanogens relative to acetoclastic methanogens was consistent with syntrophic acetate oxidation measured in methanogenic crude oil degrading enrichment cultures. qPCR of the Methanomicrobiales indicated growth characteristics consistent with measured rates of methane production and growth in partnership with Smithella.
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影响因子：
4.4
作者：
Ashelford, KE;Chuzhanova, NA;Weightman, AJ
通讯作者：
Weightman, AJ
影响因子：
5
作者：
Auburger, G;Winter, J
通讯作者：
Winter, J
影响因子：
4.4
作者：
Ashelford, Kevin E.;Chuzhanova, Nadia A.;Weightman, Andrew J.
通讯作者：
Weightman, Andrew J.
影响因子：
4.4
作者：
EDWARDS, EA;GRBICGALIC, D
通讯作者：
GRBICGALIC, D
DOI：
10.1007/s10295-006-0095-2
发表时间：
2006-06-01
影响因子：
3.4
作者：
Dunsmore, Braden;Youldon, James;Vance, Ian
通讯作者：
Vance, Ian