Aggrecan in bovine tendon.
Aggrecan in bovine tendon.
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牛腱中的聚集蛋白聚糖。
DOI：
10.1016/0945-053x(94)90006-x
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Robbins,JR
中科院分区：
文献类型：
--
作者：
Vogel,KG;Sandy,JD;Pogány,G;Robbins,JR
Large proteoglycans were purified by ion-exchange chromatography, gel filtration and CsCl gradient centrifugation from the compressed and tensional regions of adult bovine deep flexor tendon. Tryptic peptide maps of proteoglycan from the compressed region were very similar to maps of aggrecan from bovine articular cartilage, with evidence for the presence of all fifteen previously identified markers from the G1, G2 and G3 domains. The presence of aggrecan in these samples was confirmed by sequencing the G1 peptide YPIHTPR. The equivalent maps for large proteoglycan from tensional tendon were also consistent with the presence of aggrecan, and this was confirmed by sequencing three marker peptides from each of the G2 and G3 domains. However, G1 marker peptides were conspicuously absent from tensional samples. Northern blots for aggrecan mRNA showed high levels in cells from compressed tendon and articular cartilage. Extended exposure revealed a lower level of hybridization to RNA from tensional tendon as well. The results confirm that aggrecan, which is similar in core protein structure to articular cartilage aggrecan, is the predominant chondroitin sulfate-bearing large proteoglycan of compressed tendon. The results also indicate that aggrecan fragments lacking the G1 domain can account for the small amounts of chondroitin sulfate-bearing large proteoglycan in tensional regions of adult tendon.
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成纤维细胞培养物的蛋白多糖合成是从承受不同机械力的成年牛肌腱区域开始的。
影响因子：
6.6
作者：
Vogel,KG;Keller,EJ;Lenhoff,RJ;Campbell,K;Koob,TJ
通讯作者：
Koob,TJ
从 cDNA 克隆推导出的牛软骨蛋白多糖的部分氨基酸序列包含许多以同源重复排列的 Ser-Gly 序列。
DOI：
10.1042/bj2430255
发表时间：
1987
期刊：
The Biochemical journal
影响因子：
--
作者：
A. Oldberg;P. Antonsson;D. Heinegård
通讯作者：
D. Heinegård
影响因子：
4.1
作者：
HEINEGARD, D;BJORNEPERSSON, A;VOGEL, K
通讯作者：
VOGEL, K
影响因子：
4.1
作者：
GILLARD, GC;MERRILEES, MJ;FLINT, MH
通讯作者：
FLINT, MH
牛软骨蛋白聚糖富含硫酸角质素的区域由连续重复的六肽基序组成。
DOI：
10.1016/s0021-9258(18)71603-7
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Antonsson;D. Heinegård;Åke Oldberg
通讯作者：
Åke Oldberg