Enhanced bone formation by transforming growth factor-beta1-releasing collagen/chitosan microgranules.
Enhanced bone formation by transforming growth factor-beta1-releasing collagen/chitosan microgranules.
复制标题
通过转化释放生长因子-β1 的胶原蛋白/壳聚糖微粒增强骨形成。
DOI：
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
Seungjin Lee
中科院分区：
文献类型：
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作者：
J. Lee;Kyoung;Seung;I. Rhyu;Yong;Yoon‐Jeong Park;C. Chung;Seungjin Lee
以胶原/壳聚糖复合微粒作为骨替代材料，以获得高的成骨效能。微粒具有以紧密堆积填充各种类型的缺损部位的灵活性。相互连通的孔隙在微粒之间形成空间，这允许新骨长入和血管化。此外，将转化生长因子-β 1（TGF-β 1）掺入微粒中，以提高骨愈合功效。通过将混合溶液滴入NaOH/乙醇溶液中来制备胶原/壳聚糖微粒。通过将微粒浸泡在TGF-β 1溶液中，将TGF-β 1加载到胶原/壳聚糖微粒中。进行扫描电子显微镜（SEM）观察和实验，检查TGF-β 1从壳聚糖和胶原/壳聚糖微粒的释放。使用SEM检查微粒上的细胞形态，并使用溴化二甲基噻唑四唑测定法评价细胞增殖。通过测量碱性磷酸酶（ALP 3）活性以及检测骨钙素测定来评估分化的细胞功能。利用兔颅骨缺损模型进行体内骨再生实验。TGF-β 1以治疗浓度从胶原/壳聚糖微粒中释放4周。扫描电镜显示，成骨细胞与骨微粒紧密结合，呈多层增殖。在所测试的不同类型的微粒中，成骨细胞在负载TGF-β 1的微粒上的增殖最高。在培养过程中，所有样品的ALP 3活性和骨钙素水平均升高，并且负载TGF-β 1的微粒具有显著高于其他微粒的ALP 3活性和骨钙素含量。在兔颅骨缺损中，负载TGF-β 1的微粒在4周后比未负载TGF-β 1的微粒表现出更高的骨再生能力。
Collagen/chitosan composite microgranules were fabricated as bone substitutes for the purpose of obtaining high bone-forming efficacy. The microgranules have the flexibility to fill various types of defect sites with closer packing. The interconnected pores formed spaces between the microgranules, which allowed new bone ingrowth and vascularization. In addition, the transforming growth factor-beta 1 (TGF-beta1) was incorporated into the microgranules in order to improve bone-healing efficacy. The collagen/chitosan microgranules were fabricated by dropping a mixed solution into a NaOH/ethanol solution. TGF-beta1 was loaded into the collagen/chitosan microgranules by soaking the microgranules in a TGF-beta1 solution. Scanning electron microscopy (SEM) observations and experiments examining the release of TGF-beta1 from chitosan and the collagen/chitosan microgranules were performed. SEM was used to examine the cell morphologies on the microgranules and cell proliferation was evaluated using a dimethylthiazole tetrazolium bromide assay. The differentiated cell function was assessed by measuring the alkaline phosphatase (ALPase) activity as well as detecting an osteocalcin assay. The in vivo bone-regeneration experiments were performed using a rabbit calvarial defect model. TGF-beta1 was released from the collagen/chitosan microgranules at a therapeutic concentration for 4 weeks. SEM indicated that the seeded osteoblastic cells were firmly attached to the microgranules and proliferated in a multilayer manner. The proliferation of the osteoblasts on the TGF-beta1-loaded microgranules was the highest among the different types of microgranules tested. The ALPase activity and osteocalcin level of all the samples increased during the culture period, and the TGF-beta1-loaded microgranules had a significantly higher ALPase activity and osteocalcin content than the other microgranules. The TGF-beta1-loaded microgranules demonstrated a higher bone-regenerative capacity in the rabbit calvarial defects after 4 weeks than the TGF-beta1-unloaded microgranules.
影响因子：
14
作者：
Boyan, BD;Hummert, TW;Schwartz, Z
通讯作者：
Schwartz, Z
影响因子：
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作者：
Shea, LD;Wang, D;Mooney, DJ
通讯作者：
Mooney, DJ