A distinct type of sialyl Lewis X antigen defined by a novel monoclonal antibody is selectively expressed on helper memory T cells.
A distinct type of sialyl Lewis X antigen defined by a novel monoclonal antibody is selectively expressed on helper memory T cells.
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由新型单克隆抗体定义的一种独特类型的唾液酸路易斯 X 抗原在辅助记忆 T 细胞上选择性表达。
DOI：
10.1182/blood.v82.9.2797.2797
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发表时间：
1993
期刊：
影响因子：
20.3
通讯作者：
Reiji Kannagi
中科院分区：
文献类型：
--
作者：
Katsuyuki Ohmori;Akiko Takada;lchiroh Ohwaki;Naofumi Takahashi;Yoshitaka Furukawa;Michiyuki Maeda;M. Kiso;A. Hasegawa;Mari Kannagi;Reiji Kannagi
已知人辅助记忆T细胞的一个亚群粘附于在精氨酸激活的内皮细胞上表达的E-选择素。然而，唾液酸Lex抗原，E-选择素的碳水化合物配体，在这些细胞上几乎检测不到，至少当典型的抗唾液酸Lex抗体用于检测时。我们针对化学合成的唾液酸Lex糖脂制备物提出的MoAb之一（2F 3，IgM）被发现选择性地与健康个体外周淋巴细胞中的CD 4 + CD 45 RObright + CD 45 RA-辅助记忆T细胞反应。该抗体的特异性与迄今报道的典型抗唾液酸Lex抗体FH-6和SNH-3的特异性形成鲜明对比。已知这些经典的抗唾液酸Lex抗体与自然杀伤（NK）细胞的亚群反应，但如果健康个体的静息外周T或B细胞未被活化，则不与所述细胞的任何特定亚群反应。另一方面，新产生的2F 3抗体与辅助记忆T细胞特异性反应，而不与NK细胞、B细胞或除辅助记忆T细胞以外的任何T细胞反应。当针对唾液酸Lex活性糖脂抗原进行测试时，2F 3的反应性与经典的抗唾液酸Lex抗体的反应性没有显著差异。但是，当针对从细胞糖蛋白制备的寡糖进行测试时，2F 3检测到一组独特的O-连接寡糖，其对经典的抗唾液酸Lex抗体不反应。我们的研究结果表明，各种分子种类的唾液酸Lex抗原存在于细胞糖蛋白的碳水化合物侧链上，并且辅助记忆T细胞表达由2F 3定义的不同类型的唾液酸Lex抗原，但不能被其他典型的抗唾液酸Lex抗体有效地检测到。在培养的淋巴细胞白血病细胞中，成人T细胞白血病（ATL）细胞优先表达2F 3定义的抗原，而急性淋巴细胞白血病细胞很少表达该抗原。表达2F 3定义的抗原的培养的ATL细胞显示出对细胞因子激活的内皮细胞的明显的E-选择素依赖性粘附，并且2F 3定义的唾液酸Lex抗原充当E-选择素的配体，如通过与2F 3抗体的粘附的明显抑制所确定的。（400字处截断摘要）
A subset of human helper memory T cells is known to adhere to E-selectin expressed on cytokine-activated endothelial cells. However, sialyl Lex antigen, the carbohydrate ligand for E-selectin, has been hardly detectable on these cells, at least when typical anti-sialyl Lex antibodies were used for detection. One of the MoAbs (2F3, IgM), which we raised against a chemically synthesized sialyl Lex glycolipid preparation, is found to react selectively to CD4+ CD45RObright+ CD45RA- helper memory T cells among peripheral lymphocytes in healthy individuals. The specificity of the antibody is in clear contrast to that of the hitherto reported typical anti-sialyl Lex antibodies FH-6 and SNH-3. These classical anti-sialyl Lex antibodies were known to react to a subset of natural killer (NK) cells, but were not reactive with any particular subset of resting peripheral T or B cells of healthy individuals if the cells were not activated. On the other hand, the newly generated 2F3 antibody specifically reacted to helper memory T cells, and did not react to NK cells, B cells, or any T cells other than helper memory T cells. When tested against the sialyl Lex-active glycolipid antigen, the reactivity of 2F3 was not significantly different from that of the classical anti-sialyl Lex antibodies. But when tested against oligosaccharides prepared from cellular glycoproteins, 2F3 detected a distinct set of O-linked oligosaccharides, which were not reactive to the classical anti-sialyl Lex antibodies. Our results suggest that various molecular species of sialyl Lex antigens are present on carbohydrate side chains of cellular glycoproteins, and that helper memory T cells express a distinct type of sialyl Lex antigen that is defined by 2F3 but is not efficiently detected by other typical anti-sialyl Lex antibodies. Among cultured lymphocytic leukemia cells, the adult T-cell leukemia (ATL) cells preferentially expressed the 2F3-defined antigen, and acute lymphocytic leukemia cells rarely expressed the antigen. The cultured ATL cells expressing the 2F3-defined antigen showed a clear E-selectin-dependent adhesion to cytokin-activated endothelial cells, and the 2F3-defined sialyl Lex antigen served as a ligand for E-selectin as ascertained by the clear inhibition of adhesion with the 2F3 antibody.(ABSTRACT TRUNCATED AT 400 WORDS)
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影响因子：
56.9
作者：
BEVILACQUA, MP;STENGELIN, S;SEED, B
通讯作者：
SEED, B
DOI：
--
发表时间：
1992-12
期刊：
The American journal of pathology
影响因子：
--
作者：
J. Munro;S. Lo;C. Corless;M. Robertson;N. Lee;R. Barnhill;D. Weinberg;M. Bevilacqua
通讯作者：
J. Munro;S. Lo;C. Corless;M. Robertson;N. Lee;R. Barnhill;D. Weinberg;M. Bevilacqua
通过阻断内皮细胞和血小板的 O-糖基化来抑制选择素依赖性肿瘤细胞与这些细胞的粘附。
DOI：
10.1016/0006-291x(92)91872-n
发表时间：
1992
影响因子：
3.1
作者：
Kojima,N;Handa,K;Newman,W;Hakomori,S
通讯作者：
Hakomori,S
DOI：
--
发表时间：
1990
期刊：
The American journal of pathology
影响因子：
--
作者：
L. Picker;S. Michie;L. Rott;E. Butcher
通讯作者：
L. Picker;S. Michie;L. Rott;E. Butcher
影响因子：
56.9
作者：
WALZ, G;ARUFFO, A;SEED, B
通讯作者：
SEED, B