Utility of SAM68 in the progression and prognosis for bladder cancer.
Utility of SAM68 in the progression and prognosis for bladder cancer.
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SAM68 在膀胱癌进展和预后中的效用
DOI：
10.1186/s12885-015-1367-x
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发表时间：
2015-05-06
期刊：
影响因子：
3.8
通讯作者：
Zhou F
中科院分区：
文献类型：
--
作者：
Zhang Z;Yu C;Li Y;Jiang L;Zhou F
肌肉浸润性膀胱癌（MIBC）通常是致命的，非MIBC（NMIBC）可以复发和进展，但目前预后标志物不足。SAM 68是RNA结合蛋白的成员，据报道有助于其他癌症的进展。本研究的目的是探讨SAM 68在膀胱癌进展和预后中的潜在效用。采用定量PCR和免疫组化方法检测10对MIBC和邻近正常膀胱尿路上皮组织以及8对同一患者的MIBC和非MIBC（NMIBC）组织中SAM 68的表达。此外，SAM68蛋白的表达水平和定位在129个临床病理特征的MIBC通过免疫组化检测。预后相关性通过纳入标准预后因素的多变量分析确定。SAM68的表达在MIBC组织中与相邻的正常膀胱尿路上皮相比升高，并且在MIBC组织中与同一患者的NMIBC组织相比在转录和翻译水平上都增加。对于MIBC，SAM 68的高表达和核质共表达与较高的T分期、较高的N分期和较差的无复发生存率相关。SAM68低表达和高表达的MIBC患者的5年无复发生存率分别为80%和52.9%（p = 0.001）。SAM 68核质共表达的5年无复发生存率（49.2%）低于SAM 68仅表达于细胞核（82.5%）或细胞质（75.5%）。多因素分析显示，SAM 68表达水平、核质共表达、T分期、N分期均为影响MIBC患者无复发生存率的独立预后因素。与正常尿路上皮和NMIBC相比，MIBC中SAM 68表达增加，并且似乎是MIBC的潜在有用的预后标志物。
Muscle invasive bladder cancer (MIBC) is often lethal and non-MIBC (NMIBC) can recur and progress, yet prognostic markers are currently inadequate. SAM68, a member of RNA-binding proteins, has been reported to contribute to progression of other cancers. The aim of this study is to investigate the potential utility of SAM68 in the progression and prognosis of bladder cancer. Quantitative PCR and immunohistochemistry were utilized to examine the expression of SAM68 in ten pairs of MIBC and adjacent normal bladder urothelium, and eight pairs of MIBC and non-MIBC (NMIBC) tissues from the same patient. Moreover, SAM68 protein expression level and localization were examined by immunohistochemistry in 129 clinicopathologically characterized MIBC samples. Prognostic associations were determined by multivariable analysis incorporating standard prognostic factors. SAM68 expression was elevated in MIBC tissues compared with adjacent normal bladder urothelium, and was increased at both transcriptional and translational levels in MIBC tissues compared with NMIBC tissues of the same patient. For MIBC, high expression and nucleus-cytoplasm co-expression of SAM68 were associated with higher T-stage, higher N-stage and worse recurrence-free survival. Five-year recurrence-free survival was 80% and 52.9% for MIBC patients with low and high SAM68 expression, respectively (p = 0.001). SAM68 nucleus-cytoplasm co-expression associated with worse 5-year recurrence-free survival rate (49.2%) than SAM68 expression confined to the nucleus (82.5%) or cytoplasm (75.5%) alone. On multivariable analysis SAM68 expression level, SAM68 nucleus-cytoplasm co-expression, T-stage, and N-stage were all independent prognostic factors for recurrence-free survival of MIBC patients. SAM68 expression is increased in MIBC when compared to normal urothelium and NMIBC, and appears to be a potentially useful prognostic marker for MIBC.
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影响因子：
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作者：
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