The mechanism of TiaoGanYiPi formula for treating chronic hepatitis B by network pharmacology and molecular docking verification.
The mechanism of TiaoGanYiPi formula for treating chronic hepatitis B by network pharmacology and molecular docking verification.
复制标题
调肝益脾方治疗慢性乙型肝炎的作用机制通过网络药理学和分子对接验证。
DOI：
10.1038/s41598-021-87812-9
复制
发表时间：
2021-04-16
影响因子：
4.6
通讯作者：
Ye Y
中科院分区：
文献类型：
--
作者：
Cao X;Zao X;Xue B;Chen H;Zhang J;Li S;Li X;Zhu S;Guo R;Li X;Ye Y
中药调肝益脾方对由B型肝炎病毒（HBV）感染引起的慢性B型肝炎（CH B）有较好的疗效。因此，我们的目的是阐明TGYP和CHB之间的机制和潜在的靶点。从公共数据库中获得TGYP的活性化合物和相关推定靶标以及CHB的疾病靶标。通过网络构建和模块分析，确定了TGYP和CHB之间的关键目标。在基因表达综合数据集（GEO）和正常肝细胞系LO 2中检测关键靶点的表达。我们首先获得了11个主要富集在癌症、细胞周期和HBV相关通路中的关键靶点。在GSE 83148数据库中验证了关键靶点的表达与HBV感染和肝脏炎症相关。实时荧光定量PCR和CCK-8法检测结果表明，TGYP能调节细胞中CCNA 2、ABL 1、CDK 4、CDKN 1A、IGFR和MAP 2K 1等关键靶点的表达，促进LO 2细胞增殖。结论：本研究确定了TGYP治疗慢性乙型肝炎的活性成分和关键作用靶点，发现TGYP可能通过促进肝细胞增殖和抑制肝脏炎症过程而发挥治疗慢性乙型肝炎的作用。
The Chinese herbal formula TiaoGanYiPi (TGYP) showed effective against chronic hepatitis B (CHB) caused by hepatitis B virus (HBV) infection. Hence, we aimed to clarify the mechanisms and potential targets between TGYP and CHB. The active compounds and related putative targets of TGYP, and disease targets of CHB were obtained from the public databases. The key targets between TGYP and CHB were identified through the network construction and module analysis. The expression of the key targets was detected in Gene Expression Omnibus (GEO) dataset and normal hepatocyte cell line LO2. We first obtained 11 key targets which were predominantly enriched in the Cancer, Cell cycle and HBV-related pathways. And the expression of the key targets was related to HBV infection and liver inflammation verified in GSE83148 database. Furthermore, the results of real-time quantitative PCR and CCK-8 assay indicated that TGYP could regulate the expression of key targets including CCNA2, ABL1, CDK4, CDKN1A, IGFR and MAP2K1, and promote proliferation of LO2 cells. In coclusion, we identified the active compounds and key targets btween TGYP and CHB, and found that the TGYP might exhibite curative effect on CHB via promoting hepatocyte proliferation and inhibiting the liver inflammatory processes.
登录
查看更多内容
影响因子：
5.1
作者：
Nelson NP;Easterbrook PJ;McMahon BJ
通讯作者：
McMahon BJ
影响因子：
2.7
作者：
Guo L;Liang Y
通讯作者：
Liang Y
影响因子：
6.6
作者：
Li, Xiaoke;Zhou, Daqiao;Ye, Yong'an
通讯作者：
Ye, Yong'an
影响因子：
168.9
作者：
Schweitzer, Aparna;Horn, Johannes;Ott, Joerdis J.
通讯作者：
Ott, Joerdis J.
影响因子：
5.1
作者：
Lin, YL;Shen, CC;Chang, YY
通讯作者：
Chang, YY