Effects of the chinese traditional prescription xiaoyaosan decoction on chronic immobilization stress-induced changes in behavior and ultrastructure in rat hippocampus.
Effects of the chinese traditional prescription xiaoyaosan decoction on chronic immobilization stress-induced changes in behavior and ultrastructure in rat hippocampus.
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DOI：
10.1155/2013/984797
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Yue GX
中科院分区：
文献类型：
--
作者：
Liang Y;Guo XL;Chen JX;Yue GX
逍遥散作为治疗应激、抑郁相关疾病的传统药物，在中国家已有几千年的历史。本研究的目的。目的：观察XYS汤在慢性束缚应激诱导的大鼠海马神经元突触可塑性相关的α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic酸(AMPA)受体上的抗抑郁作用的可能机制。材料和方法动物随机分为5组：(1)对照组；(2)假手术组；(3)脑缺血再灌流21d组；(4)醒脑散治疗组；(5)6-氰基-7-硝基-2，3-二酮(CNQX)阳性组，单侧杏仁核注射竞争性谷氨酸受体拮抗剂CNQX。连续用药21d后，测定大鼠的旷场实验(OPT)和高架正迷宫实验(EPM)，用电子显微镜观察海马CA1区的超微结构，用实时荧光定量聚合酶链式反应(Real-time qPCR)检测海马CA1区AMPA受体亚单位GluR1和GluR2的mRNA水平。结果。CIS组大鼠在中心区的活动时间增加，在OPT的总行走距离减少。在EPM中，他们还显示出中心区时间和入射次数减少，张开手臂时间和入射次数减少，而闭合手臂时间增加。同时观察大鼠海马CA1区超微结构的损伤。XYS汤组和CNQX组大鼠行为学改变和海马CA1区超微结构损伤均有明显改善；XYS汤组还能逆转CIS诱导的CA1区GluR2和CNQX区GluR2mRNA表达的下降和GluR1mRNA表达的升高。结论。醒脑散可有效地产生抗抑郁药样作用，其机制可能与AMPA受体相关的海马区突触可塑性有关。
Xiaoyaosan (XYS) decoction has been widely used as a traditional medicine for treating stress and depression-related disorders in China for thousands of years. Aim of the Study. To observe the potential mechanism of XYS decoction's antidepressant-like effect in α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors related to synaptic plasticity in the hippocampus rats induced by chronic immobilization stress (CIS). Materials and Methods. Animals were randomly divided into five groups: (1) control group; (2) sham-operated group; (3) CIS group, in which rats were conducted CIS for 21 days; (4) XYS decoction treatment group; (5) 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) positive group, in which the amygdala of CIS rats was unilaterally microinjected with a competitive glutamate receptor antagonist, CNQX. After CIS for 21 days, the open field test (OPT) and elevated plus-maze test (EPM) were measured, the ultrastructure of hippocampus CA1 subregion was observed by the electron microscopy; both the GluR1 and GluR2 mRNA level of AMPA receptor subunits in hippocampus CA1 subregion were detected by real-time qPCR. Results. Rats subjected to CIS exhibited increases in time in central zone and decreases in total distance traveled in the OPT. In the EPM, they also showed decreases in center zone time and entries, open arm time and entries, and an increase in close arm time. Ultrastructural damage in the hippocampus CA1 was also observed. XYS decoction and CNQX showed significant improvement behavioral changes and the ultrastructural damage of the hippocampus CA1; XYS decoction also reversed CIS-induced decreases in GluR2 mRNA and increases in GluR1 mRNA in the hippocampus CA1 as well as CNQX. Conclusions. XYS decoction may effectively produce an antidepressant-like effect, which appears to be involved AMPA receptors related synaptic plasticity of hippocampus.
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影响因子：
16.2
作者：
Greger, IH;Khatri, L;Ziff, EB
通讯作者：
Ziff, EB
影响因子：
4.2
作者：
OLNEY, JW
通讯作者：
OLNEY, JW
DOI：
10.1155/2013/509134
发表时间：
2013
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Cui HZ;Wang LM;Zhao X;Liu YY;Wang SX;Li XH;Jiang YM;Chen JX
通讯作者：
Chen JX
DOI：
10.1155/2012/154271
发表时间：
2012
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Jiang YM;Meng ZZ;Yue GX;Chen JX
通讯作者：
Chen JX
影响因子：
2
作者：
AGGLETON, JP
通讯作者：
AGGLETON, JP