Root defense analysis against Fusarium oxysporum reveals new regulators to confer resistance.
Root defense analysis against Fusarium oxysporum reveals new regulators to confer resistance.
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DOI：
10.1038/srep05584
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发表时间：
2014-07-07
影响因子：
4.6
通讯作者：
Schenk PM
中科院分区：
文献类型：
--
作者：
Chen YC;Wong CL;Muzzi F;Vlaardingerbroek I;Kidd BN;Schenk PM
尖孢镰刀菌是一种侵染根部的真菌病原菌，能在包括拟南芥在内的多种植物上引起枯萎病。对该病原菌的防御反应的研究主要是利用叶片组织进行的，对根的防御反应知之甚少。在本研究中，我们利用基因芯片分析了尖孢镰刀菌侵染后根基因的表达情况。与叶片反应相反，根组织没有表现出强烈的防御相关基因表达的诱导，而是显示出更大比例的抑制基因。从微阵列中差异表达的基因中筛选插入突变体发现了转录因子乙烯反应因子72(ERF72)在尖孢镰刀菌易感性中的作用。由于ERF72在抑制细胞程序性死亡和解毒活性氧(ROS)方面的作用，我们检测了pub22/pub23/pub24 U-box类型E3泛素连接酶三重突变体，该突变体具有增强的ROS产生，以响应病原体的攻击。我们发现pub22/23/24突变体对尖孢镰刀菌的感染具有更强的抵抗力，这表明增强的先天免疫反应提供了对尖孢镰刀菌的保护。我们的结论是，根介导的防御土壤传播的病原体可以在多个水平上提供。
Fusarium oxysporum is a root-infecting fungal pathogen that causes wilt disease on a broad range of plant species, including Arabidopsis thaliana. Investigation of the defense response against this pathogen had primarily been conducted using leaf tissue and little was known about the root defense response. In this study, we profiled the expression of root genes after infection with F. oxysporum by microarray analysis. In contrast to the leaf response, root tissue did not show a strong induction of defense-associated gene expression and instead showed a greater proportion of repressed genes. Screening insertion mutants from differentially expressed genes in the microarray uncovered a role for the transcription factor ETHYLENE RESPONSE FACTOR72 (ERF72) in susceptibility to F. oxysporum. Due to the role of ERF72 in suppressing programmed cell death and detoxifying reactive oxygen species (ROS), we examined the pub22/pub23/pub24 U-box type E3 ubiquitin ligase triple mutant which is known to possess enhanced ROS production in response to pathogen challenge. We found that the pub22/23/24 mutant is more resistant to F. oxysporum infection, suggesting that a heightened innate immune response provides protection against F. oxysporum. We conclude that root-mediated defenses against soil-borne pathogens can be provided at multiple levels.
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