Functional Characterization of Two PLP-Dependent Enzymes Involved in Capsular Polysaccharide Biosynthesis from Campylobacter jejuni.
Functional Characterization of Two PLP-Dependent Enzymes Involved in Capsular Polysaccharide Biosynthesis from Campylobacter jejuni.
复制标题
DOI：
10.1021/acs.biochem.1c00439
复制
发表时间：
2021-09-21
期刊：
影响因子：
2.9
通讯作者：
Raushel FM
中科院分区：
文献类型：
--
作者：
Riegert AS;Narindoshvili T;Coricello A;Richards NGJ;Raushel FM
空肠弯曲杆菌是一种革兰氏阴性致病菌，可引起弯曲杆菌病，一种胃肠炎。C.空肠是世界上最常见的食源性疾病，超过沙门氏菌和大肠杆菌。杆菌将C.空肠是一层糖分子，称为荚膜多糖，在C.空肠NCTC 11168由d-甘油-l-葡庚糖、d-葡糖醛酸、d-N-乙酰基-半乳糖胺和d-核糖的重复单元组成。d-葡萄糖醛酸部分进一步用丝氨醇或乙醇胺酰胺化。目前还不清楚这些修饰是如何合成和连接到多糖。在这里，我们报告的催化活性的两个以前未表征的，PLP依赖酶，Cj1436和Cj1437，从C。空肠NCTC 11168。使用质谱和核磁共振的组合，我们确定Cj1436催化L-丝氨酸磷酸脱羧为乙醇胺磷酸。Cj1437被证明在L-谷氨酸存在下催化磷酸二羟丙酮转氨为（S）-丝氨酸磷酸。本文还讨论了C.空肠进行了讨论。
Campylobacter jejuni is a Gram-negative, pathogenic bacterium which causes campylobacteriosis, a form of gastroenteritis. C. jejuni is the most frequent cause of food borne illness in the world, surpassing Salmonella and E. coli. Coating the surface of C. jejuni is a layer of sugar molecules known as the capsular polysaccharide that, in C. jejuni NCTC 11168, is composed of a repeating unit of d-glycero-l-gluco-heptose, d-glucuronic acid, d-N-acetyl-galactosamine, and d-ribose. The d-glucuronic acid moiety is further amidated with either serinol or ethanolamine. It is unknown how these modifications are synthesized and attached to the polysaccharide. Here we report the catalytic activities of two previously uncharacterized, PLP-dependent enzymes, Cj1436 and Cj1437, from C. jejuni NCTC 11168. Using a combination of mass spectrometry and nuclear magnetic resonance, we determined that Cj1436 catalyzes the decarboxylation of l-serine phosphate to ethanolamine phosphate. Cj1437 was shown to catalyze the transamination of dihydroxyacetone phosphate to (S)-serinol phosphate in the presence of l-glutamate. The probable routes to the ultimate formation of the glucuronamide substructures in the capsular polysaccharides of C. jejuni are discussed.
登录
查看更多内容
影响因子：
5.7
作者：
Guerry P;Poly F;Riddle M;Maue AC;Chen YH;Monteiro MA
通讯作者：
Monteiro MA
DOI：
10.1016/j.bbapap.2015.04.015
发表时间：
2015-08
影响因子：
3.2
作者：
Gerlt, John A.;Bouvier, Jason T.;Davidson, Daniel B.;Imker, Heidi J.;Sadkhin, Boris;Slater, David R.;Whalen, Katie L.
通讯作者：
Whalen, Katie L.
影响因子：
2.9
作者：
Huddleston, Jamison P.;Raushel, Frank M.
通讯作者：
Raushel, Frank M.
影响因子：
4.8
作者：
Bieberich, E;Kawaguchi, T;Yu, RK
通讯作者：
Yu, RK
影响因子：
4.8
作者：
McNally, David J.;Lamoureux, Marc P.;Szymanski, Christine M.
通讯作者：
Szymanski, Christine M.