Fine-scale linkage mapping reveals a small set of candidate genes influencing honey bee grooming behavior in response to Varroa mites.
Fine-scale linkage mapping reveals a small set of candidate genes influencing honey bee grooming behavior in response to Varroa mites.
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DOI：
10.1371/journal.pone.0047269
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Hunt GJ
中科院分区：
文献类型：
--
作者：
Arechavaleta-Velasco ME;Alcala-Escamilla K;Robles-Rios C;Tsuruda JM;Hunt GJ
北美的蜜蜂种群已经经历了15-20年的高年度群体死亡率。许多养蜂业研究人员认为，引入的被称为瓦螨（V. destructor）的寄生虫是导致蜂群死亡的最重要因素。限制螨种群在蜂群中增长的一个重要抵抗机制是某些蜜蜂品系将螨从其身体中清除的能力。为了寻找影响这一特性的基因，我们使用Illumina Bead Station基因分型阵列确定了一个家族中数百只工蜂的基因型，其中超过1000个单核苷酸多态性显然是影响这种行为的等位基因分离的。连锁分析提供了包含1,313个标记的基因组序列遗传图谱。分析了基因型与梳理行为的关系，测量了个体蜜蜂在将螨虫放在胸前后开始梳理的时间。定量性状-位点间隔图谱在5号染色体上发现了一个在全染色体水平上显著的单染色体区域（p<0.05）， LOD评分为2.72。数量性状位点定位的95%置信区间仅包含27个基因（蜜蜂官方基因注释集2），包括Atlastin、Ataxin和Neurexin-1 (AmNrx1)，这些基因具有潜在的神经发育和行为作用。atlasatin和Ataxin同源物与人类神经系统疾病有关。AmNrx1编码一种突触前蛋白，该蛋白具有许多选择性剪接的同种异构体。Neurexin-1影响大脑突触的生长、维持和成熟，以及突触中最重要的受体类型。Neurexin-1还与人类的自闭症谱系障碍和精神分裂症以及小鼠的自我梳理行为有关。
Populations of honey bees in North America have been experiencing high annual colony mortality for 15–20 years. Many apicultural researchers believe that introduced parasites called Varroa mites (V. destructor) are the most important factor in colony deaths. One important resistance mechanism that limits mite population growth in colonies is the ability of some lines of honey bees to groom mites from their bodies. To search for genes influencing this trait, we used an Illumina Bead Station genotyping array to determine the genotypes of several hundred worker bees at over a thousand single-nucleotide polymorphisms in a family that was apparently segregating for alleles influencing this behavior. Linkage analyses provided a genetic map with 1,313 markers anchored to genome sequence. Genotypes were analyzed for association with grooming behavior, measured as the time that individual bees took to initiate grooming after mites were placed on their thoraces. Quantitative-trait-locus interval mapping identified a single chromosomal region that was significant at the chromosome-wide level (p<0.05) on chromosome 5 with a LOD score of 2.72. The 95% confidence interval for quantitative trait locus location contained only 27 genes (honey bee official gene annotation set 2) including Atlastin, Ataxin and Neurexin-1 (AmNrx1), which have potential neurodevelopmental and behavioral effects. Atlastin and Ataxin homologs are associated with neurological diseases in humans. AmNrx1 codes for a presynaptic protein with many alternatively spliced isoforms. Neurexin-1 influences the growth, maintenance and maturation of synapses in the brain, as well as the type of receptors most prominent within synapses. Neurexin-1 has also been associated with autism spectrum disorder and schizophrenia in humans, and self-grooming behavior in mice.
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影响因子：
1.9
作者：
Dahle, Bjorn
通讯作者：
Dahle, Bjorn
影响因子：
3.7
作者：
Biswas S;Reinhard J;Oakeshott J;Russell R;Srinivasan MV;Claudianos C
通讯作者：
Claudianos C
影响因子：
16.2
作者：
Choi YB;Li HL;Kassabov SR;Jin I;Puthanveettil SV;Karl KA;Lu Y;Kim JH;Bailey CH;Kandel ER
通讯作者：
Kandel ER
DOI：
10.1002/ajmg.b.31063
发表时间：
2010-06-05
影响因子：
2.8
作者：
Ching, Michael S. L.;Shen, Yiping;Tan, Wen-Hann;Jeste, Shafali S.;Morrow, Eric M.;Chen, Xiaoli;Mukaddes, Nahit M.;Yoo, Seung-Yun;Hanson, Ellen;Hundley, Rachel;Austin, Christina;Becker, Ronald E.;Berry, Gerard T.;Driscoll, Katherine;Engle, Elizabeth C.;Friedman, Sandra;Gusella, James F.;Hisama, Fuki M.;Irons, Mira B.;Lafiosca, Tina;LeClair, Elaine;Miller, David T.;Neessen, Michael;Picker, Jonathan D.;Rappaport, Leonard;Rooney, Cynthia M.;Sarco, Dean P.;Stoler, Joan M.;Walsh, Christopher A.;Wolff, Robert R.;Zhang, Ting;Nasir, Ramzi H.;Wu, Bai-Lin
通讯作者：
Wu, Bai-Lin
DOI：
10.1101/sqb.2003.68.69
发表时间：
2003-01-01
期刊：
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY
影响因子：
--
作者：
Fan, JB;Oliphant, A;Chee, MS
通讯作者：
Chee, MS