Sensory regulation of neuroligins and neurexin I in the honeybee brain.
Sensory regulation of neuroligins and neurexin I in the honeybee brain.
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DOI：
10.1371/journal.pone.0009133
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发表时间：
2010-02-09
期刊：
影响因子：
3.7
通讯作者：
Claudianos C
中科院分区：
文献类型：
--
作者：
Biswas S;Reinhard J;Oakeshott J;Russell R;Srinivasan MV;Claudianos C
最近与人类自闭症等神经疾病有关的Neurexins和Neurliins是一种高度保守的黏附蛋白，发现于神经元的突触膜上。这些结合伙伴产生一个跨突触的桥梁，促进突触的成熟和规范。人们认为，在出生后发育的大脑中，存在一种引导突触形成的神经尿蛋白和神经连接蛋白相互作用的最佳时空编码。因此，我们使用具有高级感觉处理、学习和记忆能力的行为模型系统蜜蜂，研究了神经连接蛋白和神经毒素是否受到感觉输入的差异调节。在三种不同的行为范式：感觉剥夺、联合嗅觉学习和偏侧感觉输入中，通过qRT-PCR分析来评估神经连接素1-5(NLG1-5)和神经尿素I(NrxI)的全脑表达水平。与蜂箱蜜蜂相比，感觉剥夺蜜蜂的NLG1表达水平较低，但NLG2-5和NrxI的表达水平普遍升高。与未经训练的对照蜜蜂相比，接受过联想气味训练的蜜蜂显著增加了NrxI、NLG1和NLG3的表达水平。与对照蜜蜂相比，在触角截断后将感觉输入偏侧的蜜蜂显示出NLG1表达的特定增加，这种情况只会随着时间的推移而发生。我们的结果表明：(1)感觉剥夺过程中缺少突触修剪；(2)NLG1的表达随着感觉刺激的增加而增加；(3)伴随着基因表达的变化，NrxI与所有神经连接蛋白相互作用；(4)有证据表明，偏侧损伤后存在突触补偿。
Neurexins and neuroligins, which have recently been associated with neurological disorders such as autism in humans, are highly conserved adhesive proteins found on synaptic membranes of neurons. These binding partners produce a trans-synaptic bridge that facilitates maturation and specification of synapses. It is believed that there exists an optimal spatio-temporal code of neurexin and neuroligin interactions that guide synapse formation in the postnatal developing brain. Therefore, we investigated whether neuroligins and neurexin are differentially regulated by sensory input using a behavioural model system with an advanced capacity for sensory processing, learning and memory, the honeybee. Whole brain expression levels of neuroligin 1–5 (NLG1–5) and neurexin I (NrxI) were estimated by qRT-PCR analysis in three different behavioural paradigms: sensory deprivation, associative scent learning, and lateralised sensory input. Sensory deprived bees had a lower level of NLG1 expression, but a generally increased level of NLG2–5 and NrxI expression compared to hive bees. Bees that had undergone associative scent training had significantly increased levels of NrxI, NLG1 and NLG3 expression compared to untrained control bees. Bees that had lateralised sensory input after antennal amputation showed a specific increase in NLG1 expression compared to control bees, which only happened over time. Our results suggest that (1) there is a lack of synaptic pruning during sensory deprivation; (2) NLG1 expression increases with sensory stimulation; (3) concomitant changes in gene expression suggests NrxI interacts with all neuroligins; (4) there is evidence for synaptic compensation after lateralised injury.
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DOI：
10.1002/neu.10215
发表时间：
2003-07-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
Devaud, JM;Acebes, A;Ferrús, A
通讯作者：
Ferrús, A
影响因子：
2.5
作者：
Funada, M;Yasuo, S;Kadowaki, T
通讯作者：
Kadowaki, T
DOI：
10.1002/neu.10256
发表时间：
2003-11-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
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作者：
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通讯作者：
Robinson, GE
影响因子：
4.8
作者：
Comoletti, D;Flynn, R;Taylor, P
通讯作者：
Taylor, P
影响因子：
64.8
作者：
EHRET, G
通讯作者：
EHRET, G