Specialized odorant receptors in social insects that detect cuticular hydrocarbon cues and candidate pheromones.

Specialized odorant receptors in social insects that detect cuticular hydrocarbon cues and candidate pheromones.
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DOI:
10.1038/s41467-017-00099-1
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发表时间:
2017-08-17
影响因子:
16.6
通讯作者:
Ray A
Ray A
中科院分区:
综合性期刊1区
文献类型:
--
作者:
Pask GM;Slone JD;Millar JG;Das P;Moreira JA;Zhou X;Bello J;Berger SL;Bonasio R;Desplan C;Reinberg D;Liebig J;Zwiebel LJ;Ray A

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真社会性昆虫利用表皮碳氢化合物作为信息素的成分,介导社会行为,如种姓和巢友识别,以及生殖调节。在像Harpegnathos saltator这样的蚂蚁中,女王产生一种抑制工人卵巢发育的信息素,如果她被移除,工人可以过渡到一种被称为gamergate的生殖状态。在这里,我们的功能特征的气味受体(ORS)的一个亚家族的九个外显子的基因结构,已经经历了大规模的扩张,在蚂蚁和其他真社会性昆虫。我们去角质化22个代表性的成员,发现他们可以检测到表皮碳氢化合物从不同的蚂蚁种姓,与一个(HsOr263),强烈响应gamergate提取物和候选皇后信息素成分。系统测试后,与不同的面板的碳氢化合物,我们发现,大多数Harpegnathos saltator Ors是狭隘的调整,这表明,几个受体必须有助于检测和区分不同的表皮碳氢化合物重要的介导真社会行为。表皮碳氢化合物(CHC)介导真社会性昆虫个体间的相互作用,但CHC的感觉受体尚不清楚。在这里,作者表明,在蚂蚁,如H。saltator是气味受体(HsOrs)的9个外显子亚家族,对CHC产生反应,果蝇神经元中HsOrs的异位表达赋予了对CHC的反应性。
Eusocial insects use cuticular hydrocarbons as components of pheromones that mediate social behaviours, such as caste and nestmate recognition, and regulation of reproduction. In ants such as Harpegnathos saltator, the queen produces a pheromone which suppresses the development of workers’ ovaries and if she is removed, workers can transition to a reproductive state known as gamergate. Here we functionally characterize a subfamily of odorant receptors (Ors) with a nine-exon gene structure that have undergone a massive expansion in ants and other eusocial insects. We deorphanize 22 representative members and find they can detect cuticular hydrocarbons from different ant castes, with one (HsOr263) that responds strongly to gamergate extract and a candidate queen pheromone component. After systematic testing with a diverse panel of hydrocarbons, we find that most Harpegnathos saltator Ors are narrowly tuned, suggesting that several receptors must contribute to detection and discrimination of different cuticular hydrocarbons important in mediating eusocial behaviour. Cuticular hydrocarbons (CHC) mediate the interactions between individuals in eusocial insects, but the sensory receptors for CHCs are unclear. Here the authors show that in ants such as H. saltator, the 9-exon subfamily of odorant receptors (HsOrs) responds to CHCs, and ectopic expression of HsOrs in Drosophila neurons imparts responsiveness to CHCs.
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发表时间: 2014-03
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