Wavelength censoring for spectroscopy in optical functional neuroimaging.
Wavelength censoring for spectroscopy in optical functional neuroimaging.
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光学功能神经成像中光谱学的波长审查。
DOI：
10.1088/1361-6560/abd418
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发表时间：
2021-03-12
影响因子：
3.5
通讯作者：
Yodh AG
中科院分区：
文献类型：
--
作者：
White BR;Padawer-Curry JA;Ko T;Baker W;Breimann J;Cohen AS;Licht DJ;Yodh AG
光学神经监测提供了对神经血管生理学和大脑结构和功能的深入了解。这些方法依赖于光谱学，将光吸收变化与生理发色团（如氧合血红蛋白和脱氧血红蛋白）浓度的变化联系起来。在临床或临床前实践中，不同波长的数据质量可能会有很大差异。在这种情况下，标准的光谱方法可能表现不佳，导致数据丢失和限制视场。为了解决这个问题，从而提高光学神经监测的鲁棒性，我们开发，在这篇手稿中，新的方法来执行光谱，即使数据质量表现出波长依赖的空间变化。我们试图了解空间，基于波长的删失对血红蛋白光谱的生理准确性和实用性的影响。我们分析的原则是相当普遍的，但为了使方法有形，我们专注于小鼠静息状态功能连接的光学固有信号成像。从使用四个源的光谱开始，所有可能的子集光谱矩阵进行了理论评估，使用模拟数据，并使用实验数据。将这些结果与使用全光谱矩阵进行比较，以确定哪些子集产生稳健的结果。我们的研究结果表明，准确计算血红蛋白浓度的变化和由此产生的功能连接网络地图是可能的，即使在某些波长的审查。此外，我们发现，使用总血红蛋白（而不是氧合血红蛋白或脱氧血红蛋白）的变化产生的结果对实验噪音更稳健，并允许保存更多的数据。这种新的和严格的图像处理方法应提高临床和临床前功能神经影像学研究的保真度。
Optical neuromonitoring provides insight into neurovascular physiology and brain structure and function. These methods rely on spectroscopy to relate light absorption changes to variation of concentrations of physiologic chromophores such as oxy- and deoxyhemoglobin. In clinical or preclinical practice, data quality can vary significantly across wavelengths. In such situations, standard spectroscopic methods may perform poorly, resulting in data loss and limiting field-of-view. To address this issue, and thereby improve the robustness of optical neuromonitoring, we develop, in this manuscript, novel methods to perform spectroscopy even when data quality exhibits wavelength-dependent spatial variation. We sought to understand the impact of spatial, wavelength-based censoring on the physiologic accuracy and utility of hemoglobin spectroscopy. The principles of our analysis are quite general, but to make the methodology tangible we focused on optical intrinsic signal imaging of resting-state functional connectivity in mice. Starting with spectroscopy using four sources, all possible subset spectroscopy matrices were assessed theoretically, using simulated data, and using experimental data. These results were compared against the use of the full spectroscopy matrix to determine which subsets yielded robust results. Our results demonstrated that accurate calculation of changes in hemoglobin concentrations and the resulting functional connectivity network maps was possible even with censoring of some wavelengths. Additionally, we found that the use of changes in total hemoglobin (rather than oxy- or deoxyhemoglobin) yielded results more robust to experimental noise and allowed for the preservation of more data. This new and rigorous image processing method should improve the fidelity of clinical and preclinical functional neuroimaging studies.
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影响因子：
5.7
作者：
Eggebrecht, Adam T.;White, Brian R.;Ferradal, Silvina L.;Chen, Chunxiao;Zhan, Yuxuan;Snyder, Abraham Z.;Dehghani, Hamid;Culver, Joseph P.
通讯作者：
Culver, Joseph P.
DOI：
10.1523/jneurosci.5845-11.2012
发表时间：
2012-03-28
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Bero AW;Bauer AQ;Stewart FR;White BR;Cirrito JR;Raichle ME;Culver JP;Holtzman DM
通讯作者：
Holtzman DM
影响因子：
3.5
作者：
Okui, N;Okada, E
通讯作者：
Okada, E
影响因子：
3
作者：
Silasi, Gergely;Xiao, Dongsheng;Murphy, Timothy H.
通讯作者：
Murphy, Timothy H.
影响因子：
3.5
作者：
Brendel, Bernhard;Nielsen, Tim
通讯作者：
Nielsen, Tim