Establishment of an inducing medium for type III effector secretion in Xanthomonas campestris pv. campestris.
Establishment of an inducing medium for type III effector secretion in Xanthomonas campestris pv. campestris.
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野油菜黄单胞菌 III 型效应子分泌诱导培养基的建立。
DOI：
10.1590/s1517-83822013000300045
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Tang JL
中科院分区：
文献类型：
--
作者：
Jiang GF;Jiang BL;Yang M;Liu S;Liu J;Liang XX;Bai XF;Tang DJ;Lu GT;He YQ;Yu DQ;Tang JL
众所周知，III型分泌系统（T3 SS）和III型（T3）效应子对于大多数细菌植物病原体的致病性是必需的，并且T3 SS和T3效应子的表达在丰富培养基中被抑制，但在基本培养基和植物中被诱导。为了促进对T3 SS和T3效应子的深入研究，关键是建立T3效应子表达和分泌的培养基。油菜黄单胞菌campestris（Xcc）是研究植物-病原菌互作的模式细菌。迄今为止，尚未确定用于Xcc T3效应子分泌的培养基。在这里，我们比较了四种基本培养基（MME，MMX，XVM 2和XOM 2），据报道，它们在黄单胞菌属中诱导T3表达。并发现MME对于Xcc T3效应物的表达和分泌最有效。通过对MME的碳源、氮源和pH值的优化，建立了Xcc T3效应子分泌能力比MME强3倍左右的XCM 1培养基。我们进一步优化了XCM 1中磷酸盐、钙和镁的浓度，发现镁浓度较低的XCM 1（重命名为XCM 2）的效率约为XCM 1的10倍（同时，约为MME的30倍）。因此，我们建立了诱导培养基XCM 2，其优选用于Xcc中的T3效应子分泌。
It is well known that the type III secretion system (T3SS) and type III (T3) effectors are essential for the pathogenicity of most bacterial phytopathogens and that the expression of T3SS and T3 effectors is suppressed in rich media but induced in minimal media and plants. To facilitate in-depth studies on T3SS and T3 effectors, it is crucial to establish a medium for T3 effector expression and secretion. Xanthomonas campestris pv. campestris (Xcc) is a model bacterium for studying plant-pathogen interactions. To date no medium for Xcc T3 effector secretion has been defined. Here, we compared four minimal media (MME, MMX, XVM2, and XOM2) which are reported for T3 expression induction in Xanthomonas spp. and found that MME is most efficient for expression and secretion of Xcc T3 effectors. By optimization of carbon and nitrogen sources and pH value based on MME, we established XCM1 medium, which is about 3 times stronger than MME for Xcc T3 effectors secretion. We further optimized the concentration of phosphate, calcium, and magnesium in XCM1 and found that XCM1 with a lower concentration of magnesium (renamed as XCM2) is about 10 times as efficient as XCM1 (meanwhile, about 30 times stronger than MME). Thus, we established an inducing medium XCM2 which is preferred for T3 effector secretion in Xcc.
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影响因子：
3.2
作者：
Wengelnik, K;Marie, C;Bonas, U
通讯作者：
Bonas, U
比较和功能基因组学揭示了植物病原体黄单胞菌参考菌株和大量中国分离株之间的遗传多样性和宿主特异性的决定因素。油菜。
影响因子：
12.3
作者：
He, Yong-Qiang;Zhang, Liang;Jiang, Bo-Le;Zhang, Zheng-Chun;Xu, Rong-Qi;Tang, Dong-Jie;Qin, Jing;Jiang, Wei;Zhang, Xia;Liao, Jie;Cao, Jin-Ru;Zhang, Sui-Sheng;Wei, Mei-Liang;Liang, Xiao-Xia;Lu, Guang-Tao;Feng, Jia-Xun;Chen, Baoshan;Cheng, Jing;Tang, Ji-Liang
通讯作者：
Tang, Ji-Liang
DOI：
10.1073/pnas.83.22.8447
发表时间：
1986-11-01
影响因子：
11.1
作者：
JEFFERSON, RA;BURGESS, SM;HIRSH, D
通讯作者：
HIRSH, D
影响因子：
2.6
作者：
Jiang, Bo-Le;He, Yong-Qiang;Tang, Ji-Liang
通讯作者：
Tang, Ji-Liang
影响因子：
3.2
作者：
Schechter, LM;Roberts, KA;Collmer, A
通讯作者：
Collmer, A