Comparative and functional genomics reveals genetic diversity and determinants of host specificity among reference strains and a large collection of Chinese isolates of the phytopathogen Xanthomonas campestris pv. campestris.
Comparative and functional genomics reveals genetic diversity and determinants of host specificity among reference strains and a large collection of Chinese isolates of the phytopathogen Xanthomonas campestris pv. campestris.
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比较和功能基因组学揭示了植物病原体黄单胞菌参考菌株和大量中国分离株之间的遗传多样性和宿主特异性的决定因素。油菜。
DOI：
10.1186/gb-2007-8-10-r218
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发表时间：
2007
期刊：
影响因子：
12.3
通讯作者：
Tang, Ji-Liang
中科院分区：
文献类型：
--
作者：
He, Yong-Qiang;Zhang, Liang;Jiang, Bo-Le;Zhang, Zheng-Chun;Xu, Rong-Qi;Tang, Dong-Jie;Qin, Jing;Jiang, Wei;Zhang, Xia;Liao, Jie;Cao, Jin-Ru;Zhang, Sui-Sheng;Wei, Mei-Liang;Liang, Xiao-Xia;Lu, Guang-Tao;Feng, Jia-Xun;Chen, Baoshan;Cheng, Jing;Tang, Ji-Liang
基于野油菜黄单胞菌 pv.campestris (Xcc) 基因组的微阵列的构建，以及用于分析其他 18 种强毒力 Xcc 菌株的分析，揭示了对 Xcc 菌株的遗传多样性和宿主特异性的决定因素的见解。野油菜黄单胞菌（Xanthomonascampestrispathovarcampestris，Xcc）是全世界十字花科植物黑腐病的病原体。 Xcc 的分子遗传多样性和宿主特异性尚不清楚。我们基于Xcc菌株8004的完整基因组序列构建了微阵列，并通过基于阵列的18个强毒菌株的比较基因组杂交分析研究了Xcc的遗传多样性和宿主特异性。结果表明，阵列上点样的 4,186 个编码序列 (CDS) 中包含 3,405 个的遗传核心是保守的，并且具有 730 个 CDS 的灵活基因库不存在/高度分歧 (AHD)。结果还显示，304 个已证实/推测的致病基因中有 258 个是保守的，其中 46 个是 AHD。保守的致病基因主要包括涉及I、II和III型分泌系统、群体感应系统、胞外酶和多糖产生的基因，以及许多其他已证实的致病基因，而AHD CDS包含编码IV型分泌系统（T4SS）和III型效应子的基因。 Xcc T4SS 缺失突变体表现出与野生型相同的毒力。此外，还鉴定了三个无毒基因（avrXccC、avrXccE1 和 avrBs1）。 avrXccC和avrXccE1分别对寄主芥菜品种光头和大白菜品种中白83赋予无毒力，avrBs1对非寄主辣椒ECW10R赋予过敏反应。大约 80% 的 Xcc CDS，包括 258 个已证实/推测的致病性基因，在不同菌株中是保守的。 Xcc T4SS 不参与致病性。开发了一种从 AHD 基因中识别决定宿主特异性的 avr 基因的有效策略。
Construction of a microarray based on the genome of Xanthomonas campestris pv.campestris (Xcc), and its use to analyse 18 other virulent Xcc strains, revealed insights into the genetic diversity and determinants of host specificity of Xcc strains. Xanthomonas campestris pathovar campestris (Xcc) is the causal agent of black rot disease of crucifers worldwide. The molecular genetic diversity and host specificity of Xcc are poorly understood. We constructed a microarray based on the complete genome sequence of Xcc strain 8004 and investigated the genetic diversity and host specificity of Xcc by array-based comparative genome hybridization analyses of 18 virulent strains. The results demonstrate that a genetic core comprising 3,405 of the 4,186 coding sequences (CDSs) spotted on the array are conserved and a flexible gene pool with 730 CDSs is absent/highly divergent (AHD). The results also revealed that 258 of the 304 proved/presumed pathogenicity genes are conserved and 46 are AHD. The conserved pathogenicity genes include mainly the genes involved in type I, II and III secretion systems, the quorum sensing system, extracellular enzymes and polysaccharide production, as well as many other proved pathogenicity genes, while the AHD CDSs contain the genes encoding type IV secretion system (T4SS) and type III-effectors. A Xcc T4SS-deletion mutant displayed the same virulence as wild type. Furthermore, three avirulence genes (avrXccC, avrXccE1 and avrBs1) were identified. avrXccC and avrXccE1 conferred avirulence on the hosts mustard cultivar Guangtou and Chinese cabbage cultivar Zhongbai-83, respectively, and avrBs1 conferred hypersensitive response on the nonhost pepper ECW10R. About 80% of the Xcc CDSs, including 258 proved/presumed pathogenicity genes, is conserved in different strains. Xcc T4SS is not involved in pathogenicity. An efficient strategy to identify avr genes determining host specificity from the AHD genes was developed.
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DOI：
10.1073/pnas.93.25.14648
发表时间：
1996-12-10
影响因子：
11.1
作者：
Censini, S;Lange, C;Covacci, A
通讯作者：
Covacci, A
影响因子：
3.2
作者：
ALVAREZ, AM;BENEDICT, AA;GABRIEL, DW
通讯作者：
GABRIEL, DW
DOI：
10.1073/pnas.132393999
发表时间：
2002-09-17
影响因子：
11.1
作者：
Bhattacharyya, A;Stilwagen, S;Kyrpides, NC
通讯作者：
Kyrpides, NC
影响因子：
4.1
作者：
Büttner, D;Noël, L;Bonas, U
通讯作者：
Bonas, U
影响因子：
3.5
作者：
Castañeda, A;Reddy, JD;Gabriel, DW
通讯作者：
Gabriel, DW