A modular detector for flow cytometric multicolor fluorescence measurements.
A modular detector for flow cytometric multicolor fluorescence measurements.
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用于流式细胞术多色荧光测量的模块化检测器。
DOI：
10.1002/cyto.990080403
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Stewart,CC
中科院分区：
文献类型：
--
作者：
Steinkamp,JA;Habbersett,RC;Stewart,CC
A modular detector for measuring multicolor fluorescence from cells illuminated by single or multiple lasers has been developed for flow cytometers. Motion picture projector, camera, and CCTV/video lenses were evaluated for use in the detector by comparing their physical characteristics, image quality, and light collection efficiencies. A 25‐mm focal length F/0.95 CCTV lens was selected, based on our critera and test results. The detector was constructed out of square aluminum extrusion channels. A CCTV lens mounted on the outside of the first channel collected light emitted from cells and collimated it towards filters and secondary CCTV lenses located in each channel. The secondary lenses functioned as relay optics for directing and focusing light onto pinhole spatial filters for measurement by photomultipliers. The detector design allowed any number of channels to be connected together and the versatility for making simultaneous or sequential measurements. Measurements on lymphocytes labeled with four monoclonal antibodies conjugated to fluorescent dyes and measurements on viable tumor cells stained for DNA content and with three fluorescent‐labeled antibodies were used to demonstrate the detector's capabilities.
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影响因子：
3.2
作者：
DEAN, PN;PINKEL, D
通讯作者：
PINKEL, D
Cytomat-R：计算机控制的多激光源多参数流式细胞光度计系统。
DOI：
10.1177/25.7.330733
发表时间：
1977
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
H. Shapiro;E. Schildkraut;R. Curbelo;R. Turner;R. Webb;D. C. Brown;M. Block
通讯作者：
M. Block
影响因子：
1.8
作者：
G. Salzman;J. M. Crowell;J. Martin;T. T. Trujillo-T.;A. Romero;P. Mullaney;P. M. Labauve
通讯作者：
G. Salzman;J. M. Crowell;J. Martin;T. T. Trujillo-T.;A. Romero;P. Mullaney;P. M. Labauve
用于流式细胞术和分选的模块化电子器件：LACEL 系统。
DOI：
10.1002/cyto.990010506
发表时间：
1981
期刊：
Cytometry
影响因子：
--
作者：
R. Hiebert;J. Jett;G. Salzman
通讯作者：
G. Salzman
生物细胞的快速多种质量成分分析
影响因子：
5.2
作者：
L. Kamentsky;M. Melamed
通讯作者：
M. Melamed