Aspergillus oxylipin signaling and quorum sensing pathways depend on g protein-coupled receptors.
Aspergillus oxylipin signaling and quorum sensing pathways depend on g protein-coupled receptors.
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曲霉素信号传导和群体感应途径取决于G蛋白偶联受体。
DOI：
10.3390/toxins4090695
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发表时间：
2012-09
期刊：
影响因子：
4.2
通讯作者：
Keller NP
中科院分区：
文献类型：
--
作者：
Affeldt KJ;Brodhagen M;Keller NP
氧脂素调节曲霉菌的发育和霉菌毒素的产生，并且还参与曲霉菌的群体感应机制。尽管对这些氧脂素是如何合成的以及它们调节哪些过程有广泛的了解，但对于真菌如何检测和传递这些信号却一无所知。据推测，G蛋白偶联受体（GPCR）参与其中，因为它们是哺乳动物中已知的氧脂素受体，并且在曲霉菌中已经鉴定出许多假定的GPCR。在此，我们提供的证据表明，氧脂素刺激构巢曲霉中cAMP的爆发，并且构巢曲霉的一种GPCR（gprD）的缺失会阻止这种cAMP的积累。黄曲霉在不同密度下生长时会经历氧脂素介导的发育转变，这会调节孢子、菌核和黄曲霉毒素的产生。黄曲霉编码两个假定的GprD同源物，GprC和GprD，并且我们在此证明它们是向高密度发育状态转变以及对高密度培养的废弃培养基作出反应所必需的。发现调节生存结构（菌核）、接种体（孢子）和黄曲霉毒素产生的GPCR，为未来抗真菌治疗方法的开发带来了希望。
Oxylipins regulate Aspergillus development and mycotoxin production and are also involved in Aspergillus quorum sensing mechanisms. Despite extensive knowledge of how these oxylipins are synthesized and what processes they regulate, nothing is known about how these signals are detected and transmitted by the fungus. G protein-coupled receptors (GPCR) have been speculated to be involved as they are known oxylipin receptors in mammals, and many putative GPCRs have been identified in the Aspergilli. Here, we present evidence that oxylipins stimulate a burst in cAMP in A. nidulans, and that loss of an A. nidulans GPCR, gprD, prevents this cAMP accumulation. A. flavus undergoes an oxylipin-mediated developmental shift when grown at different densities, and this regulates spore, sclerotial and aflatoxin production. A. flavus encodes two putative GprD homologs, GprC and GprD, and we demonstrate here that they are required to transition to a high-density development state, as well as to respond to spent medium of a high-density culture. The finding of GPCRs that regulate production of survival structures (sclerotia), inoculum (spores) and aflatoxin holds promise for future development of anti-fungal therapeutics.
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影响因子：
2.9
作者：
Albuquerque P;Casadevall A
通讯作者：
Casadevall A
影响因子：
14.9
作者：
Goujon M;McWilliam H;Li W;Valentin F;Squizzato S;Paern J;Lopez R
通讯作者：
Lopez R
影响因子：
4.4
作者：
Brown, S. Horowitz;Zarnowski, R.;Keller, N. P.
通讯作者：
Keller, N. P.
影响因子：
3.5
作者：
Brown, Sigal Horowitz;Scott, James B.;Keller, Nancy P.
通讯作者：
Keller, Nancy P.
影响因子：
3.3
作者：
Lafon, A;Seo, JA;d'Enfert, C
通讯作者：
d'Enfert, C