Analysis of microRNA expression in the prepubertal testis.
Analysis of microRNA expression in the prepubertal testis.
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DOI：
10.1371/journal.pone.0015317
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发表时间：
2010-12-29
期刊：
影响因子：
3.7
通讯作者：
Matzuk MM
中科院分区：
文献类型：
--
作者：
Buchold GM;Coarfa C;Kim J;Milosavljevic A;Gunaratne PH;Matzuk MM
在D. melanogaster和小鼠;然而，通过Dicer突变导致miRNA功能条件性丧失导致两个物种中减数分裂前生殖细胞增殖缺陷。这突出了miRNAs在早期精子发生过程中潜在的重要但未表征的作用。本研究的目的是在出生后第7、10和14天表征小鼠睾丸miRNAs的内容和编辑，其主要来自精原细胞和精母细胞，与成年小鼠睾丸中miRNAs的先前描述相反，其主要反映精子细胞的内容。先前的研究表明，在出生后第14天，小鼠睾丸中存在大量的miRNA;然而，通过对B6;129背景下的睾丸进行下一代测序，我们发现了大量的miRNA早期表达，并描述了在此期间miRNA特征的变化。我们在出生后第14天的睾丸中检测到X染色体上编码的miRNA的稳健表达，与先前的研究一致，表明它们对减数分裂性染色体失活具有抗性。出乎意料的是，我们还发现了出生后第14天2号染色体上的大多数miRNA和出生后第7天12号染色体上的大多数miRNA的相似位置富集。我们定量了3种类型的miRNA变异的体内发育变化，包括5′异质性、编辑和3′核苷酸添加。我们确定了11个推定的新型青春期睾丸miRNAs，其发育表达表明可能在早期男性生殖细胞发育中发挥作用。这些研究为解释与睾丸病理学相关的miRNA变化和鉴定睾丸中miRNA编辑机制的新组分提供了基础。
Only thirteen microRNAs are conserved between D. melanogaster and the mouse; however, conditional loss of miRNA function through mutation of Dicer causes defects in proliferation of premeiotic germ cells in both species. This highlights the potentially important, but uncharacterized, role of miRNAs during early spermatogenesis. The goal of this study was to characterize on postnatal day 7, 10, and 14 the content and editing of murine testicular miRNAs, which predominantly arise from spermatogonia and spermatocytes, in contrast to prior descriptions of miRNAs in the adult mouse testis which largely reflects the content of spermatids. Previous studies have shown miRNAs to be abundant in the mouse testis by postnatal day 14; however, through Next Generation Sequencing of testes from a B6;129 background we found abundant earlier expression of miRNAs and describe shifts in the miRNA signature during this period. We detected robust expression of miRNAs encoded on the X chromosome in postnatal day 14 testes, consistent with prior studies showing their resistance to meiotic sex chromosome inactivation. Unexpectedly, we also found a similar positional enrichment for most miRNAs on chromosome 2 at postnatal day 14 and for those on chromosome 12 at postnatal day 7. We quantified in vivo developmental changes in three types of miRNA variation including 5′ heterogeneity, editing, and 3′ nucleotide addition. We identified eleven putative novel pubertal testis miRNAs whose developmental expression suggests a possible role in early male germ cell development. These studies provide a foundation for interpretation of miRNA changes associated with testicular pathology and identification of novel components of the miRNA editing machinery in the testis.
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影响因子：
3.7
作者：
Creighton CJ;Benham AL;Zhu H;Khan MF;Reid JG;Nagaraja AK;Fountain MD;Dziadek O;Han D;Ma L;Kim J;Hawkins SM;Anderson ML;Matzuk MM;Gunaratne PH
通讯作者：
Gunaratne PH
DOI：
10.1073/pnas.0509333103
发表时间：
2006-02-21
影响因子：
11.1
作者：
Kotaja, N;Bhattacharyya, SN;Sassone-Corsi, P
通讯作者：
Sassone-Corsi, P
影响因子：
5.4
作者：
Gottwein, Eva;Cai, Xuezhong;Cullen, Bryan R.
通讯作者：
Cullen, Bryan R.
影响因子：
12.3
作者：
Blow MJ;Grocock RJ;van Dongen S;Enright AJ;Dicks E;Futreal PA;Wooster R;Stratton MR
通讯作者：
Stratton MR
影响因子：
14.9
作者：
Kawahara, Yukio;Megraw, Molly;Kreider, Edward;Iizasa, Hisashi;Valente, Louis;Hatzigeorgiou, Artemis G.;Nishikura, Kazuko
通讯作者：
Nishikura, Kazuko