RNA editing of human microRNAs.
RNA editing of human microRNAs.
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DOI：
10.1186/gb-2006-7-4-r27
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发表时间：
2006
期刊：
影响因子：
12.3
通讯作者：
Stratton MR
中科院分区：
文献类型：
--
作者：
Blow MJ;Grocock RJ;van Dongen S;Enright AJ;Dicks E;Futreal PA;Wooster R;Stratton MR
对来自十种人体组织的miRNA的RNA编辑的调查表明，RNA编辑增加了miRNA及其靶标的多样性。MicroRNA（miRNAs）是一种约22个核苷酸的短RNA，可调节基因表达。miRNA的初级转录物含有双链RNA，因此是腺苷至肌苷（A-to-I）RNA编辑的潜在底物。我们通过对来自同一个体的匹配基因组DNA和总cDNA的PCR产物进行序列比较，对来自10种人体组织的miRNA进行了RNA编辑的调查。在获得数据的99个miRNA转录物中，有6个（6%）在至少一个组织中进行了A-to-I编辑。7个编辑的腺苷中有4个在成熟的miRNA中，并且被预测改变3'非翻译区中的靶位点。对于另外六种miRNA，我们鉴定了来自注释的miRNA的基因组的相对链的转录物的A到I编辑。这些miRNA可能被注释到错误的基因组链上。我们的研究结果表明，RNA编辑增加了miRNA及其靶标的多样性，因此可能调节miRNA的功能。
A survey of RNA editing of miRNAs from ten human tissues indicates that RNA editing increases the diversity of miRNAs and their targets. MicroRNAs (miRNAs) are short RNAs of around 22 nucleotides that regulate gene expression. The primary transcripts of miRNAs contain double-stranded RNA and are therefore potential substrates for adenosine to inosine (A-to-I) RNA editing. We have conducted a survey of RNA editing of miRNAs from ten human tissues by sequence comparison of PCR products derived from matched genomic DNA and total cDNA from the same individual. Six out of 99 (6%) miRNA transcripts from which data were obtained were subject to A-to-I editing in at least one tissue. Four out of seven edited adenosines were in the mature miRNA and were predicted to change the target sites in 3' untranslated regions. For a further six miRNAs, we identified A-to-I editing of transcripts derived from the opposite strand of the genome to the annotated miRNA. These miRNAs may have been annotated to the wrong genomic strand. Our results indicate that RNA editing increases the diversity of miRNAs and their targets, and hence may modulate miRNA function.
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