Development and characterization of 23 polymorphic microsatellite loci for Amentotaxus argotaenia (Taxaceae), a relict vulnerable species.
Development and characterization of 23 polymorphic microsatellite loci for Amentotaxus argotaenia (Taxaceae), a relict vulnerable species.
复制标题
孑遗易危物种穗花杉（紫杉科）23 个多态性微卫星位点的开发和表征
DOI：
10.1002/aps3.1149
复制
发表时间：
2018-05
影响因子：
3.6
通讯作者：
Su Y
中科院分区：
文献类型：
--
作者：
Huang Q;Wang Z;Wang T;Su Y
以红豆杉科针叶树（Amentotaxus argotaenia）为研究对象，开发了新的微卫星标记，以研究其种群遗传变异及环境异质性对遗传结构的影响。方法与结果采用FIASCO （AFLP of Sequences COntaining repeats）快速分离技术，从argotaenia中分离得到27个微卫星位点，其中23个为多态性位点。每个位点产生1 ~ 13个等位基因和1.0 ~ 7.9个有效等位基因；观察到的杂合度和预期的杂合度分别在0.000-1.000和0.000-0.873之间变化。共有18个标记可转移到近缘种云南沙蚤。结论这些多态性标记是研究银带沙蚕群体遗传变异和局部适应潜力的宝贵遗传资源。
Premise of the Study New microsatellite markers were developed for the vulnerable conifer species Amentotaxus argotaenia (Taxaceae) to investigate population genetic variation and the effects of environmental heterogeneity on genetic structure. Methods and Results A total of 27 microsatellite loci were developed from A. argotaenia through a Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol, of which 23 were polymorphic. These markers yielded 1–13 alleles and 1.0–7.9 effective alleles per locus; levels of observed and expected heterozygosity varied from 0.000–1.000 and 0.000–0.873, respectively. In total, 18 of the markers were transferable to the related species A. yunnanensis. Conclusions These polymorphic markers are a valuable genetic resource for investigating population genetic variation and the potential for local adaptation in A. argotaenia.
登录
查看更多内容
DOI：
10.1111/j.1471-8286.2004.00684.x
发表时间：
2004-09-01
期刊：
MOLECULAR ECOLOGY NOTES
影响因子：
--
作者：
Van Oosterhout, C;Hutchinson, WF;Shipley, P
通讯作者：
Shipley, P
影响因子：
11.4
作者：
Lin, Chinsu;Chan, Ming-Hsun;Wang, Ya-Nan
通讯作者：
Wang, Ya-Nan
影响因子：
7.7
作者：
Rousset, Francois
通讯作者：
Rousset, Francois
影响因子：
3
作者：
Ho, Chin-Shang;Chen, Chaur-Tzuhn;Chiang, Yu-Chung
通讯作者：
Chiang, Yu-Chung
影响因子：
1.6
作者：
Cui, Limin;Su, Yingjuan
通讯作者：
Su, Yingjuan