Caveolae: static inpocketings of the plasma membrane, dynamic vesicles or plain artifact?
Caveolae: static inpocketings of the plasma membrane, dynamic vesicles or plain artifact?
复制标题
小凹：质膜的静态凹陷、动态囊泡还是普通伪影？
作者：
NJ Severs
The presence of small (50-90 nm diameter) vesicular invaginations (caveolae) in plasma membranes has been known since some of the earliest ultrastructural descriptions of cells, yet the nature and function of these structures continue to stimulate debate and controversy. From their association with subplasmalemmal vesicles of similar dimensions, caveolae were originally assumed to be simple pinocytotic vesicles caught in the act of pinching-off from the plasma membrane. In vascular endothelial cells, this interpretation was refined and elaborated to explain bidirectional transport of substances across the blood vessel wall. In striated and smooth muscle, on the other hand, it was replaced by the concept of specialized, semipermanent plasma membrane invaginations. The precise mechanism by which the caveola-vesicle system mediates transendothelial transport and the functional significance of muscle caveolae have been extensively discussed, often with conflicting conclusions. One of the central interpretative difficulties in the past has been the dependence on glutaraldehyde fixation to give a faithful representation of membrane organization in vivo. This limitation can now be overcome with ultrarapid freezing techniques, and the nature of caveolae has been re-examined in a number of recent studies using this approach in endothelial cells (McGuire & Twietmeyer, 1983; Robinson et al. 1984; Wagner & Andrews, 1985; Fr0kjaer-Jensen & Reese, 1986; Noguchi et al. 1986) and muscle cells (Lee et al. 1983; Frank et al. 1987; Poulos et al. 1986). The purpose of this article is to discuss briefly the impact of these findings and the questions they raise against the background of previously established concepts.
登录
查看更多内容
心房肽分泌的超微结构特征。
DOI：
10.1152/ajpheart.1986.251.2.h340
发表时间：
1986
期刊：
The American journal of physiology
影响因子：
--
作者：
Page,E;Goings,GE;Power,B;Upshaw-Earley,J
通讯作者：
Upshaw-Earley,J
DOI：
--
发表时间：
1981-08
期刊：
The American journal of pathology
影响因子：
--
作者：
E. Bonilla;K. Fischbeck;D. Schotland
通讯作者：
E. Bonilla;K. Fischbeck;D. Schotland
培养的血管内皮细胞的内吞囊泡和表面内陷：形态测量比较。
DOI：
10.1177/29.12.6172467
发表时间：
1981
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Davies,PF;Kuczera,L
通讯作者：
Kuczera,L
超快速冷冻、未经预处理、冷冻断裂心肌中的膜结构。
影响因子：
20.1
作者：
Frank,JS;Beydler,S;Mottino,G
通讯作者：
Mottino,G
用钌红作为细胞外标记物测定动脉内皮细胞中的囊泡分布。
DOI：
10.1016/s0022-5320(82)90031-4
发表时间：
1982
期刊：
Journal of ultrastructure research
影响因子：
--
作者：
Chien,S;Laufer,L;Handley,DA
通讯作者：
Handley,DA