A subnanosecond electric pulse exposure system for biological cells.
A subnanosecond electric pulse exposure system for biological cells.
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DOI：
10.1007/s11517-016-1516-7
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发表时间：
2017-07
影响因子：
3.2
通讯作者：
Schoenbach KH
中科院分区：
文献类型：
--
作者：
Xiao S;Semenov I;Petrella R;Pakhomov AG;Schoenbach KH
为研究强电场、亚纳秒脉冲对生物细胞的作用，研制了一套适用于显微镜载物台的曝光系统。该系统的通带为3GHz，能够向两个直径为100 μ m的平行钨棒（间距为170 μ m）电极提供高压电脉冲（6.2kV）。电脉冲通过π网络传递到电极，π网络用作衰减器以及阻抗匹配单元以吸收电极处的反射。通过最小化脉冲传递系统的电感，可以在显微镜下在盖玻片表面产生高达200 kV/cm的电场，脉冲持续时间为500 ps。发现盖玻片上的电场在50至70 μm的区域内均匀。在这个区域内，研究了放置在盖玻片上的神经母细胞瘤细胞由亚纳秒脉冲引起的膜电位变化。这使我们能够第一次证明细胞膜电位的去极化。
An exposure system adapted for use on a microscope stage was constructed for studying the effects of high electric field, subnanosecond pulses on biological cells. The system has a bandpass of 3 GHz and is capable of delivering high voltage electric pulses (6.2 kV) to the electrodes, which are two tungsten rods (100 μm in diameter) in parallel with a gap distance of 170 μm. Electric pulses are delivered to the electrodes through a π-network, which serves as an attenuator as well as an impedance matching unit to absorb the reflection at the electrodes. By minimizing the inductance of the pulse delivery system, it was possible to generate electric fields of up to 200 kV/cm with a pulse duration of 500 ps at the surface of the cover slip under the microscope. The electric field at the cover slip was found to be homogenous over an area of 50 to 70 μm. Within this area, neuroblastoma cells placed on the cover slip were studied with respect to membrane potential changes caused by subnanosecond pulses. This allowed us, for the first time, to demonstrate depolarization of the cell membrane potential.
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影响因子：
1.9
作者：
Schoenbach, KH;Beebe, SJ;Buescher, ES
通讯作者：
Buescher, ES
影响因子：
1.5
作者：
Schoenbach, Karl H.;Xiao, Shu;Beebe, Stephen J.
通讯作者：
Beebe, Stephen J.
影响因子：
--
作者：
Cole, KS
通讯作者：
Cole, KS
影响因子：
1.9
作者：
Pakhomov, Andrei G.;Kolb, Juergen F.;Schoenbach, Karl H.
通讯作者：
Schoenbach, Karl H.
影响因子：
1.5
作者：
Camp, J. Thomas;Jing, Yu;Schoenbach, Karl H.
通讯作者：
Schoenbach, Karl H.