Restricted Water Intake Adversely Affects Rat Vocal Fold Biology.
Restricted Water Intake Adversely Affects Rat Vocal Fold Biology.
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DOI：
10.1002/lary.28881
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发表时间：
2021-04
期刊：
影响因子：
--
通讯作者：
Sivasankar MP
中科院分区：
文献类型：
--
作者：
Duan C;do Nascimento NC;Calve S;Cox A;Sivasankar MP
对系统性脱水影响声带生物学的许多方式的整体理解仍在不断发展。也有无数的生理相关的方法来诱导全身脱水。为了解开系统性脱水对声带生物学的影响，我们需要利用现实的、临床可翻译的实验动物系统性脱水范例。限制获得水以适应临床翻译。我们研究了通过减少水分摄入而导致的全身脱水是否会对声带生物学产生负面影响。前瞻性体内研究设计。雄性Sprague Dawley大鼠（N = 13）连续5天每天给予4 mL/100 g水，而雄性对照大鼠（N = 8）给予自由饮水。安乐死后，对组织进行组织学染色、基因表达和蛋白质分析。肾脏肾素基因表达水平显著升高（P≤0.05），证实脱水。限制水分获取引起的脱水使白细胞介素-1α和粘粒素-1基因表达下调（P≤0.05）。脱水后透明质酸酶-2基因表达升高（P≤0.05）。脱水后促冻球蛋白-1蛋白水平降低（P≤0.05）。组织学分析提示限水大鼠声带透明质酸降低（P≤0.05）。每天饮水量减少5天，会破坏炎性细胞因子释放，降低质膜完整性，破坏透明质酸网络，从而损害声带生物学。这是第一个在体内模型中研究限制水分摄入对声带组织脱水影响的研究。NA（前瞻性动物研究）。
A holistic understanding of the many ways that systemic dehydration affects vocal fold biology is still evolving. There are also myriad physiologically relevant methodologies to induce systemic dehydration. To untangle the effects of systemic dehydration on vocal fold biology, we need to utilize realistic, clinically translatable paradigms of systemic dehydration in lab animals. Restricted access to water accommodates clinical translation. We investigated whether systemic dehydration via reduced water intake would negatively affect vocal fold biology. Prospective, in vivo study design. Male Sprague Dawley rats (N = 13) were provided 4 mL/100 g of water/day for 5 days, whereas male control rats (N = 8) were given ad lib access to water. Following euthanasia, tissues were processed for histological staining, gene expression, and protein assays. Renin gene expression level in kidneys increased significantly (P ≤ .05), validating dehydration. Dehydration induced by restricted water access downregulated the gene expression of interleukin-1α and desmoglein-1 (P ≤ .05). Hyaluronidase-2 gene expression increased after dehydration (P ≤ .05). The protein level of desmoglein-1 decreased after dehydration (P ≤ .05). Histological analyses suggested decreased hyaluronan (P ≤ .05) in the water-restricted rat vocal fold. Reduced daily water intake for just 5 days impairs vocal fold biology by disrupting inflammatory cytokine release, reducing plasma membrane integrity, and disrupting the hyaluronan network. This is the first study investigating the dehydrating effects of restricted water intake on vocal fold tissue in an in vivo model. NA (prospective animal study).
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