Determining the feasibility of characterising cellular senescence in human skeletal muscle and exploring associations with muscle morphology and physical function at different ages: findings from the MASS_Lifecourse Study.
Determining the feasibility of characterising cellular senescence in human skeletal muscle and exploring associations with muscle morphology and physical function at different ages: findings from the MASS_Lifecourse Study.
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DOI：
10.1007/s11357-023-00869-4
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发表时间：
2024-02
期刊：
影响因子：
5.6
通讯作者：
Sayer, Avan A.
中科院分区：
文献类型：
--
作者：
Habiballa, Leena;Hruby, Adam;Granic, Antoneta;Dodds, Richard M.;Hillman, Susan J.;Jurk, Diana;Passos, Joao F.;Sayer, Avan A.
细胞衰老可能与骨骼肌的形态学变化和身体功能随年龄的变化有关，尽管很少有人体研究。我们的目的是确定表征骨骼肌细胞衰老的可行性，并探索MASS_Lifecourse研究参与者的细胞衰老标志物，肌肉形态和身体功能之间的性别特异性关联。衰老标志物使用空间分辨方法（免疫组织化学、免疫荧光、RNA和荧光原位杂交），对40名男性和女性（年龄范围47-84岁）的肌肉活检组织进行了评估（p16、TAF（端粒相关DNA损伤灶）、HMGB 1（高迁移率族蛋白1）和核纤层蛋白B1）和形态学特征（纤维大小、数量、纤维化和中央有核纤维）。探讨了不同年龄的衰老、形态和身体功能（肌肉力量、质量和身体表现）之间的关系。我们发现，大多数衰老标志物和形态学特征与男性年龄的相关性较弱，但与女性年龄的相关性较强，尽管不显着。在女性中，衰老标志物、形态学和身体功能之间的相关性也更强：HMGB 1和握力（r = 0.52）; TAF、BMI和肌肉质量（r > 0.4）;核纤层蛋白B1和纤维化（r =-0.5）;纤维大小和肌肉质量（r ≥ 0.4）;步态速度（r =-0.5）。然而，这些关联并不显著。总之，我们已经证明，这是可行的，在人类骨骼肌细胞衰老，并探讨与不同年龄的女性和男性的形态和身体功能。这些发现需要在更大规模的研究中复制。在线版本包含补充材料，可通过10.1007/s11357-023-00869-4获得。
Cellular senescence may be associated with morphological changes in skeletal muscle and changes in physical function with age although there have been few human studies. We aimed to determine the feasibility of characterising cellular senescence in skeletal muscle and explored sex-specific associations between markers of cellular senescence, muscle morphology, and physical function in participants from the MASS_Lifecourse Study. Senescence markers (p16, TAF (Telomere-Associated DNA Damage Foci), HMGB1 (High Mobility Group Box 1), and Lamin B1) and morphological characteristics (fibre size, number, fibrosis, and centrally nucleated fibres) were assessed in muscle biopsies from 40 men and women (age range 47–84) using spatially-resolved methods (immunohistochemistry, immunofluorescence, and RNA and fluorescence in situ hybridisation). The associations between senescence, morphology, and physical function (muscle strength, mass, and physical performance) at different ages were explored. We found that most senescence markers and morphological characteristics were weakly associated with age in men but more strongly, although non-significantly, associated with age in women. Associations between senescence markers, morphology, and physical function were also stronger in women for HMGB1 and grip strength (r = 0.52); TAF, BMI, and muscle mass (r > 0.4); Lamin B1 and fibrosis (r = − 0.5); fibre size and muscle mass (r ≥ 0.4); and gait speed (r = − 0.5). However, these associations were non-significant. In conclusion, we have demonstrated that it is feasible to characterise cellular senescence in human skeletal muscle and to explore associations with morphology and physical function in women and men of different ages. The findings require replication in larger studies. The online version contains supplementary material available at 10.1007/s11357-023-00869-4.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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影响因子：
7.8
作者：
Kowald A;Passos JF;Kirkwood TBL
通讯作者：
Kirkwood TBL
影响因子：
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作者：
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