3D-Printed Tumor Phantoms for Assessment of In Vivo Fluorescence Imaging Analysis Methods.
3D-Printed Tumor Phantoms for Assessment of In Vivo Fluorescence Imaging Analysis Methods.
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DOI：
10.1007/s11307-022-01783-5
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发表时间：
2023-02
影响因子：
3.1
通讯作者：
Ruiz AJ
中科院分区：
文献类型：
--
作者：
LaRochelle EPM;Streeter SS;Littler EA;Ruiz AJ
介入荧光成像越来越多地被用于在临床和临床前模型中量化癌症生物标志物，但绝对量化因许多因素而变得复杂。多个专业组织已经建议使用光学体模对荧光引导成像系统进行定量性能评估。这个概念可以进一步扩展，以提供标准化的工具来比较和评估图像分析指标。基于实体肿瘤模型的3D打印荧光模型被开发为具有代表性的生物模拟光学特性。用包埋有固定浓度的NIR荧光团和周围材料中的零或3%非特异性荧光团的离散肿瘤产生荧光团。这些幻影首先成像的两个荧光成像系统使用两种方法的图像分割，和四个评估指标进行了计算，以证明系统性能的定量评估的变异性。然后将相同的分析技术应用于具有降低的肿瘤荧光团浓度的一个肿瘤模型。这些解剖体模模型证明了使用3D打印来制造具有广泛的降低散射（μs′：0.24-1.06 mm−1）和吸收（μa：0.005-0.14 mm−1）特性的拟人化形状的能力。体模成像和分析突出显示了与肿瘤可视化相关的测量灵敏度指标的可变性。3D打印技术提供了一个展示复杂生物模型的平台，该模型为量化荧光图像数据引入了现实世界的复杂性。这些体模设计的受控迭代开发可用作推进该领域的工具，并为荧光成像平台的性能评估之外的共识建立提供背景，并扩展对如何从成像数据中提取定量指标并在文献中报告的标准化的支持。在线版本包含补充材料，可通过10.1007/s11307-022-01783-5获得。
Interventional fluorescence imaging is increasingly being utilized to quantify cancer biomarkers in both clinical and preclinical models, yet absolute quantification is complicated by many factors. The use of optical phantoms has been suggested by multiple professional organizations for quantitative performance assessment of fluorescence guidance imaging systems. This concept can be further extended to provide standardized tools to compare and assess image analysis metrics. 3D-printed fluorescence phantoms based on solid tumor models were developed with representative bio-mimicking optical properties. Phantoms were produced with discrete tumors embedded with an NIR fluorophore of fixed concentration and either zero or 3% non-specific fluorophore in the surrounding material. These phantoms were first imaged by two fluorescence imaging systems using two methods of image segmentation, and four assessment metrics were calculated to demonstrate variability in the quantitative assessment of system performance. The same analysis techniques were then applied to one tumor model with decreasing tumor fluorophore concentrations. These anatomical phantom models demonstrate the ability to use 3D printing to manufacture anthropomorphic shapes with a wide range of reduced scattering (μs′: 0.24–1.06 mm−1) and absorption (μa: 0.005–0.14 mm−1) properties. The phantom imaging and analysis highlight variability in the measured sensitivity metrics associated with tumor visualization. 3D printing techniques provide a platform for demonstrating complex biological models that introduce real-world complexities for quantifying fluorescence image data. Controlled iterative development of these phantom designs can be used as a tool to advance the field and provide context for consensus-building beyond performance assessment of fluorescence imaging platforms, and extend support for standardizing how quantitative metrics are extracted from imaging data and reported in literature. The online version contains supplementary material available at 10.1007/s11307-022-01783-5.
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影响因子：
3.8
作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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作者：
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