Different gut microbial types were found in captive striped hamsters.
Different gut microbial types were found in captive striped hamsters.
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作者：
Fan C;Zheng Y;Xue H;Xu J;Wu M;Chen L;Xu L
分型分析已成为动物肠道微生物群落研究中对个体差异进行分类的常用方法。然而，以前对肠道微生物类型的定义更多地被理解为对不同外部干扰的被动反应过程，因为大多数研究涉及不同的环境变量。我们想知道在相同的外部环境下，动物体内是否也会出现不同的肠道微生物类型。此外，宿主性别在塑造肠道微生物群中的作用已被广泛报道;因此，本研究初步探讨了性别对潜在不同微生物类型的影响。在此，将不同性别的成年黑线仓鼠圈养在相同的受控实验室条件下，并在两个月后收集其粪便样本，以通过16 S rRNA测序评估肠道微生物群。圈养条纹仓鼠的肠道微生物群在扩增子序列变体（ASV）水平上自然地分为两种类型。Shannon指数在这两种类型之间存在显著差异。受试者工作特征（ROC）曲线显示，前30名ASV可以有效区分每种类型。线性判别分析的效果大小（LEfSe）显示富集的属乳酸杆菌，密螺旋体和Pygmaietrium在一个肠道微生物类型和富集的属Turicibacter和Ruminiclostridium在其他。前者碳水化合物代谢能力强，后者共生网络复杂，氨基酸代谢能力强。肠道微生物类型与性别无关;然而，我们确实发现某些细菌类群的相对丰度存在性别差异，包括一些特定类型的性别变异。虽然圈养动物生活在一个统一的环境中，它们的肠道细菌仍然可以分化成不同的类型，但宿主的性别在小规模圈养动物群落的分型过程中可能不起重要作用。相关的驱动因素以及其他潜在的类型需要进一步研究，以更好地了解宿主-微生物相互作用。
Typing analysis has become a popular approach to categorize individual differences in studies of animal gut microbial communities. However, previous definitions of gut microbial types were more understood as a passive reaction process to different external interferences, as most studies involve diverse environmental variables. We wondered whether distinct gut microbial types can also occur in animals under the same external environment. Moreover, the role of host sex in shaping gut microbiota has been widely reported; thus, the current study preliminarily explores the effects of sex on potential different microbial types. Here, adult striped hamsters Cricetulus barabensis of different sexes were housed under the same controlled laboratory conditions, and their fecal samples were collected after two months to assess the gut microbiota by 16S rRNA sequencing. The gut microbiota of captive striped hamsters naturally separated into two types at the amplicon sequence variant (ASV) level. There was a significant difference in the Shannon index among these two types. A receiver operating characteristic (ROC) curve showed that the top 30 ASVs could effectively distinguish each type. Linear discriminant analysis of effect size (LEfSe) showed enrichment of the genera Lactobacillus, Treponema and Pygmaiobacter in one gut microbial type and enrichment of the genera Turicibacter and Ruminiclostridium in the other. The former type had higher carbohydrate metabolism ability, while the latter harbored a more complex co-occurrence network and higher amino acid metabolism ability. The gut microbial types were not associated with sex; however, we did find sex differences in the relative abundances of certain bacterial taxa, including some type-specific sex variations. Although captive animals live in a unified environment, their gut bacteria can still differentiate into distinct types, but the sex of the hosts may not play an important role in the typing process of small-scale captive animal communities. The relevant driving factors as well as other potential types need to be further investigated to better understand host-microbe interactions.
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影响因子：
64.8
作者：
通讯作者：
--
影响因子：
5.2
作者：
通讯作者：
--
DOI：
10.1038/ismej.2015.53
发表时间：
2015-11
期刊：
The ISME journal
影响因子：
--
作者：
Maurice CF;Knowles SC;Ladau J;Pollard KS;Fenton A;Pedersen AB;Turnbaugh PJ
通讯作者：
Turnbaugh PJ
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
64.8
作者：
Arumugam, Manimozhiyan;Raes, Jeroen;Pelletier, Eric;Le Paslier, Denis;Yamada, Takuji;Mende, Daniel R.;Fernandes, Gabriel R.;Tap, Julien;Bruls, Thomas;Batto, Jean-Michel;Bertalan, Marcelo;Borruel, Natalia;Casellas, Francesc;Fernandez, Leyden;Gautier, Laurent;Hansen, Torben;Hattori, Masahira;Hayashi, Tetsuya;Kleerebezem, Michiel;Kurokawa, Ken;Leclerc, Marion;Levenez, Florence;Manichanh, Chaysavanh;Nielsen, H. Bjorn;Nielsen, Trine;Pons, Nicolas;Poulain, Julie;Qin, Junjie;Sicheritz-Ponten, Thomas;Tims, Sebastian;Torrents, David;Ugarte, Edgardo;Zoetendal, Erwin G.;Wang, Jun;Guarner, Francisco;Pedersen, Oluf;de Vos, Willem M.;Brunak, Soren;Dore, Joel;Weissenbach, Jean;Ehrlich, S. Dusko;Bork, Peer
通讯作者：
Bork, Peer