Degradation of monocrotophos by Starkeya novella YW6 isolated from paddy soil
Degradation of monocrotophos by Starkeya novella YW6 isolated from paddy soil
复制标题
稻田土中中叶霉YW6对久效磷的降解
DOI：
10.1007/s11356-015-5606-0
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发表时间：
2016-02
期刊：
影响因子：
--
通讯作者：
Qing Hong
中科院分区：
文献类型：
--
作者：
Lina Sun;Shijun Zhu;Zhengzhong Yang;Qing Chen;Hongming Liu;Jun Zhang;Gang Hu;Shunpeng Li;Qing Hong
从水稻土中分离到一株能利用久效磷（MCP）作为唯一碳源和氮源生长的细菌YW 6，经鉴定为新斯塔克亚氏菌（Starkeya novella）。菌株YW 6在36 h内完全降解0.2 mM MCP，没有任何滞后期。碳源的加入减缓了MCP的初始降解速率，而氮源的加入则促进了MCP的降解。菌株YW 6除能降解MCP外，还能降解多种含P-O-C键的有机磷农药，但不能降解含P-S-C键的乐果。根据代谢产物的产生模式和代谢产物的鉴定，提出了MCP的降解途径。MCP在P-O-C键处水解形成N-甲基乙酰乙酰胺和磷酸二甲酯; N-甲基乙酰乙酰胺转化为N-甲基-4-氧代-戊酰胺，其随后转化为5-（甲氨基）-5-氧代-戊酸，5-（甲氨基）-5-氧代-戊酸裂解为谷氨酸和甲胺。这些发现为MCP的微生物代谢提供了新的见解。据我们所知，这是第一次报道的降解MCP的斯塔克亚细菌。
A bacteria strain, YW6, capable of utilizing monocrotophos (MCP) as the sole carbon and nitrogen sources for growth was isolated from paddy soil and identified as Starkeya novella. Strain YW6 completely degraded 0.2 mM MCP within 36 h without any lag period. Addition of carbon source resulted in slowing down of the initial rate of degradation of MCP, while the presence of a more favorable source of nitrogen enhanced the degradation of MCP. In addition to the degradation of MCP, strain YW6 was also able to degrade a wide range of organophosphorus pesticides (OPs) containing P-O-C bond, but not dimethoate, which has P-S-C bond. A MCP degradation pathway was proposed on the basis of metabolite production patterns and identification of the metabolites. MCP is hydrolyzed at the P-O-C bond to form N-methylacetoacetamide and dimethyl phosphate; N-methylacetoacetamide is transformed to N-methyl-4-oxo-pentanamide, which was subsequently converted to 5-(methylamino)-5-oxo-pentanoic acid, and 5-(methylamino)-5-oxo-pentanoic acid is cleaved to glutaric acid and methylamine. These findings provide new insights into the microbial metabolism of MCP. To the best of our knowledge, this is the first report on the degradation of MCP by Starkeya bacteria.
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影响因子：
2.6
作者：
B. J. Bhadbhade;S. Sarnaik;P. Kanekar
通讯作者：
B. J. Bhadbhade;S. Sarnaik;P. Kanekar
影响因子：
4.4
作者：
Horne, I;Sutherland, TD;Oakeshott, JG
通讯作者：
Oakeshott, JG
影响因子：
4.4
作者：
D. Reasoner;E. Geldreich
通讯作者：
D. Reasoner;E. Geldreich
影响因子：
4
作者：
Bhadbhade, BJ;Sarnaik, SS;Kanekar, PP
通讯作者：
Kanekar, PP
影响因子：
2.8
作者：
S. Singh;D. Singh
通讯作者：
S. Singh;D. Singh