Three dimensional mesoscale analysis of translamellar cross-bridge morphologies in the annulus fibrosus using optical coherence tomography.
Three dimensional mesoscale analysis of translamellar cross-bridge morphologies in the annulus fibrosus using optical coherence tomography.
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DOI：
10.1002/jor.22778
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发表时间：
2015-03
影响因子：
2.8
通讯作者：
Hsieh, Adam H.
中科院分区：
文献类型：
--
作者：
Han, Sang Kuy;Chen, Chao-Wei;Wierwille, Jerry;Chen, Yu;Hsieh, Adam H.
长期以来，间盘纤维环(AF)的典型特征是由高度有序的胶原纤维组成的板层结构，这些纤维从一层到另一层以交替的斜角排列。然而，最近的一系列组织学研究表明，房颤板层包含跨越板层的富含弹性蛋白和VI型胶原的次级“交叉桥”结构。在这项研究中，我们使用光学相干断层扫描(OCT)来阐明房颤组织中这些跨板层桥的三维(3D)形态。OCT的中尺度体积图像显示了一个高度不均匀的空间网络和三维跨板层桥的分布。这一研究结果证实了跨板横桥是一种可区分的结构，它位于相邻板层之间的束间空间，并在径向上交叉于多个板层。与以前提出的从二维截面推断的模型不同，本研究的结果表明，跨层交叉桥作为一个复杂的、相互连接的网络存在。我们还发现，椎板间束间隙内的横桥长度差异更大(与本研究相比，为0.8-1.4 mm，而来自2-D切片的为0.3-0.6 mm)。基于OCT的跨板层横桥的三维形态为研究房颤结构提供了新的视角。
The defining characteristic of the annulus fibrosus (AF) of the intervertebral disc (IVD) has long been the lamellar structures that consist of highly ordered collagen fibers arranged in alternating oblique angles from one layer to the next. However, a series of recent histologic studies have demonstrated that AF lamellae contain elastin- and type VI collagen-rich secondary “cross-bridge” structures across lamellae. In this study, we use optical coherence tomography (OCT) to elucidate the three-dimensional (3D) morphologies of these translamellar cross-bridge in AF tissues. Mesoscale volumetric images by OCT reveal a highly heterogeneous spatial network and distribution of 3-D translamellar cross-bridges. The results of this study confirm the translamellar cross-bridge is identified as a distinguishable structure, which is laid in the interbundle space of adjacent lamellae and crisscrosses multiple lamellae in the radial direction. In contrast to previously proposed models extrapolated from 2-D sections, results from this current study show that translamellar cross-bridges exist as a complex, interconnected network. We also found much greater variation in lengths of cross-bridges within the interbundle space of lamellae (0.8-1.4 mm from the current study versus 0.3-0.6 mm from 2-D sections). OCT-based 3-D morphology of translamellar cross-bridge provides novel insight into the AF structure.
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影响因子：
2.4
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通讯作者：
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