The role for the exocyst complex subunits Exo70B2 and Exo70H1 in the plant-pathogen interaction.
The role for the exocyst complex subunits Exo70B2 and Exo70H1 in the plant-pathogen interaction.
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DOI：
10.1093/jxb/erq402
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发表时间：
2011-03
影响因子：
6.9
通讯作者：
Zársky V
中科院分区：
文献类型：
--
作者：
Pecenková T;Hála M;Kulich I;Kocourková D;Drdová E;Fendrych M;Toupalová H;Zársky V
最近，在植物中发现了八聚体囊泡束缚复合物外囊，并证明了其对拟南芥形态发生的重要性。与酵母和哺乳动物不同，植物中的 Exo70 胞囊亚基由多基因家族代表，在拟南芥中包含 23 个成员。对于 Exo70B2 和 Exo70H1 旁系同源物，用源自细菌延伸因子的激发子肽 elf18 处理后证实转录上调。使用酵母双杂交系统，通过 Exo70 与其他几个外囊亚基的相互作用推断出它们参与外囊复合体形成的能力。这两个基因突变的拟南芥植物被用来分析它们在接种丁香假单胞菌 pv. 后的局部反应。 maculicola 和真菌病原体 Blumeria graminis f. sp。大麦。假单胞菌敏感性测试显示，两种 exo70B2 和一种 H1 突变株系的敏感性增强。接种 Blumeria 后，在 exo70B2 突变体中发现异常乳头形成的比例增加，并具有由囊泡样结构组成的不寻常的宽晕。通过 GFP 融合测定和农杆菌介导的构建体在本塞姆氏烟草叶表皮中的瞬时表达，研究了两种 Exo70 蛋白的细胞内定位。 GFP-Exo70H1 定位在囊泡样结构中，而 GFP-Exo70B2 主要定位在细胞质中。结论：Exo70B2和Exo70H1均参与对病原体的反应，其中Exo70B2在与植物防御相关的细胞壁并置形成中具有更重要的作用。
Recently, the octameric vesicle-tethering complex exocyst was found in plants and its importance for Arabidopsis morphogenesis was demonstrated. Exo70 exocyst subunits in plants, unlike in yeasts and mammals, are represented by a multigene family, comprising 23 members in Arabidopsis. For Exo70B2 and Exo70H1 paralogues, transcriptional up-regulation was confirmed on treatment with an elicitor peptide, elf18, derived from the bacterial elongation factor. Their ability to participate in the exocyst complex formation was inferred by the interaction of both the Exo70s with several other exocyst subunits using the yeast two-hybrid system. Arabidopsis plants mutated in these two genes were used to analyse their local reaction upon inoculation with Pseudomonas syringae pv. maculicola and the fungal pathogen Blumeria graminis f. sp. hordei. The Pseudomonas sensitivity test revealed enhanced susceptibility for the two exo70B2 and one H1 mutant lines. After Blumeria inoculation, an increase in the proportion of abnormal papilla formation, with an unusual wide halo made of vesicle-like structures, was found in exo70B2 mutants. Intracellular localization of both Exo70 proteins was studied following a GFP fusion assay and Agrobacterium-mediated transient expression of the constructs in Nicotiana benthamiana leaf epidermis. GFP-Exo70H1 localizes in the vesicle-like structures, while GFP-Exo70B2 is localized mainly in the cytoplasm. It is concluded that both Exo70B2 and Exo70H1 are involved in the response to pathogens, with Exo70B2 having a more important role in cell wall apposition formation related to plant defence.
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