Discrepant expression of cytokines in inflammation- and age-related cataract patients.
Discrepant expression of cytokines in inflammation- and age-related cataract patients.
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炎症和年龄相关性白内障患者细胞因子表达的差异
DOI：
10.1371/journal.pone.0109647
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Chen W
中科院分区：
文献类型：
--
作者：
Chen W;Lin H;Zhong X;Liu Z;Geng Y;Xie C;Chen W
目的白塞氏病（BD）或伏格特-小柳-原田病（VKH）继发的炎症性白内障被认为是由细胞因子的病理性失调引起的，与年龄相关（AR）白内障不同。然而，关于细胞因子在炎症性白内障发生发展中的作用知之甚少。本研究的目的是确定炎症和年龄相关性白内障患者细胞因子表达的可能差异。方法采用抗体包被的非磁珠混合物分析技术，测定BD、VKH和AR白内障患者的细胞因子表达谱。激光闪光细胞仪（LFCM）定量测量前房房水闪光和炎性细胞。结果白细胞介素2（IL-2）、IL-4、IL-6、IL-10、IL-17 A和干扰素-γ（IFN-γ）在三种类型白内障患者的房水（AqH）、植物血凝素（PHA）刺激和非PHA刺激的外周血单个核细胞（PBMC）培养中的表达。IL-6和IFN-γ被鉴定为高于检测限，但是，在BD和VKH白内障患者中，与在AR白内障患者中观察到的水平相比，在AqH和PBMC非PHA培养物中仅IL-6的水平显著更高。相反，与VKH和AR白内障患者相比，BD白内障患者AqH中的IFN-γ显著升高。在PHA刺激的PBMC培养物中，IL-2、IFN-γ、IL-6和IL-17 A显著升高，并且VKH患者中的IL-6水平显著高于BD和AR白内障患者。LFCM检测AqH细胞因子与炎症指标的相关性分析显示，只有IL-6与炎症指标显著相关。结论炎症性白内障和AR白内障患者存在不同的细胞因子表达谱，并与体内炎症指标相关。
Purpose Inflammatory cataracts secondary to Behcet's disease (BD) or Vogt-Koyanagi-Harada disease (VKH) are thought to result from a pathological dysregulation of cytokines that is different from that of age-related (AR) cataracts. However, little is known about the function of cytokines in the development of inflammatory cataracts. The purpose of this study was to identify possible differences in cytokine expression in inflammation- and age-related cataract patients. Methods Analysis techniques involving the concomitant use of a cocktail of antibody-coated non-magnetic beads were used to determine the cytokine expression profiles of BD, VKH and AR cataract patients. Furthermore, anterior chamber aqueous flares and inflammatory cells were quantitatively measured with a laser flare cell meter (LFCM). Results The expressions of interleukin-2 (IL-2), IL-4, IL-6, IL-10, IL-17A, and interferon-γ (IFN-γ) were analyzed in aqueous humor (AqH), phytohemagglutinin (PHA)-stimulated and non-PHA-stimulated cultures of peripheral blood mononuclear cells (PBMCs) from the three types of cataract patients. IL-6 and IFN-γ were identified above the detection limits, but, among the BD and VKH cataract patients, only the levels of IL-6 were significantly higher in both the AqH and PBMC non-PHA cultures compared with the levels observed in the AR cataract patients. In contrast, IFN-γ was significantly elevated in the AqH of the BD cataract patients compared with the VKH and AR cataract patients. In the PHA-stimulated PBMC cultures, IL-2, IFN-γ, IL-6, and IL-17A were significantly increased, and the IL-6 level was significantly higher in the VKH patients than in the BD and AR cataract patients. The correlation analyses of the cytokines and inflammation indexes of the AqH obtained with the LFCM revealed that only IL-6 was significantly correlated with the inflammation index. Conclusion Distinct expression profiles of cytokines and the correlations of these profiles with in vivo inflammatory indexes for inflammatory and AR cataract patients were identified.
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DOI：
10.12659/msm.881720
发表时间：
2011-04
期刊：
Medical science monitor : international medical journal of experimental and clinical research
影响因子：
--
作者：
Hamzaoui K;Bouali E;Ghorbel I;Khanfir M;Houman H;Hamzaoui A
通讯作者：
Hamzaoui A
影响因子：
4.6
作者：
Evereklioglu, C;Er, H;Çekmen, M
通讯作者：
Çekmen, M
影响因子：
4.1
作者：
Li, B;Yang, P;Kijlstra, A
通讯作者：
Kijlstra, A
Vogt-小柳-原田病中 IL-27 表达减少与 Th17 反应增加相关
影响因子：
4.4
作者：
Wang, Chaokui;Tian, Yuan;Yang, Peizeng
通讯作者：
Yang, Peizeng
影响因子：
2
作者：
DEBOER, JH;VANHAREN, MAC;KIJLSTRA, A
通讯作者：
KIJLSTRA, A