The fungicide dodine primarily inhibits mitochondrial respiration in Ustilago maydis, but also affects plasma membrane integrity and endocytosis, which is not found in Zymoseptoria tritici.
The fungicide dodine primarily inhibits mitochondrial respiration in Ustilago maydis, but also affects plasma membrane integrity and endocytosis, which is not found in Zymoseptoria tritici.
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DOI：
10.1016/j.fgb.2020.103414
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发表时间：
2020-09
期刊：
影响因子：
--
通讯作者：
Steinberg G
中科院分区：
文献类型：
--
作者：
Schuster M;Steinberg G
杀真菌剂多丁插入玉米黑粉菌的PM中。多丁能使细胞去极化，并降低PM的流动性。maydis，但不是在Z。三个月。Dodine损害肌动蛋白斑的运动。maydis，这是由于抑制内吞作用的fimbrillium的募集受损所致。PM处的MoA是次要的，因为氧化磷酸化在低得多的多丁浓度下被抑制。真菌病原体对给定杀真菌剂的反应可能不同。早期的报道表明，两亲性杀真菌剂多丁破坏真菌质膜（PM），从而杀死这种玉米黑穗病病原体。然而，最近的一项研究在小麦病原菌Zymoseptoria triumph不支持这种作用模式（MoA）。相反，dodine抑制线粒体ATP合成，无论是在Z。tribalus和U.玉米粉这对多丁在PM的杀真菌活性提出了质疑。在这里，我们使用细胞生物学的方法，并进一步研究多丁对两种真菌质膜的影响。我们表明，多丁确实打破了在美国总理的完整性。maydis，由浓度依赖性细胞去极化指示。此外，杀真菌剂通过抑制PM处的内吞囊泡的内化来降低PM流动性并阻止内吞作用。这可能是由于受损的招聘肌动蛋白交联fimmune的内吞肌动蛋白补丁。然而，定量数据显示，对线粒体的影响代表了U.玉米粉在dodine处理的Z中没有发现这些质膜相关效应。三聚体细胞因此，抗真菌化学物质的生理作用在病原体之间可能不同。这在表征给定的杀真菌剂时值得考虑。
The fungicide dodine inserts into the PM of the corn smut fungus Ustilago maydis. Dodine depolarises cells and reduces the PM fluidity in U. maydis, but not in Z. tritici. Dodine impairs actin patch motility in U. maydis, due to impaired recruitment of fimbrin, which arrests endocytosis. The MoA at the PM is secondary, as oxidative phosphorylation is inhibited at much lower dodine concentrations. Fungal pathogens can differ in their response to a given fungicide. Early reports in the fungus Ustilago maydis suggest that the amphipathic fungicide dodine disrupts the fungal plasma membrane (PM), thereby killing this corn smut pathogen. However, a recent study in the wheat pathogen Zymoseptoria tritici does not support such mode of action (MoA). Instead, dodine inhibits mitochondrial ATP-synthesis, both in Z. tritici and U. maydis. This casts doubt on an fungicidal activity of dodine at the PM. Here, we use a cell biological approach and investigate further the effect of dodine on the plasma membrane in both fungi. We show that dodine indeed breaks the integrity of the PM in U. maydis, indicated by a concentration-dependent cell depolarization. In addition, the fungicide reduces PM fluidity and arrests endocytosis by inhibiting the internalization of endocytic vesicles at the PM. This is likely due to impaired recruitment of the actin-crosslinker fimbrin to endocytic actin patches. However, quantitative data reveal that the effect on mitochondria represents the primary MoA in U. maydis. None of these plasma membrane-associated effects were found in dodine-treated Z. tritici cells. Thus, the physiological effect of an anti-fungal chemistry can differ between pathogens. This merits consideration when characterizing a given fungicide.
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DOI：
10.1083/jcb.201505086
发表时间：
2015-12-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
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DOI：
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发表时间：
2019-07-01
影响因子：
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