A Novel UDP-Glycosyltransferase of Rhodiola crenulata Converts Tyrosol to Specifically Produce Icariside D2.
A Novel UDP-Glycosyltransferase of Rhodiola crenulata Converts Tyrosol to Specifically Produce Icariside D2.
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红景天的新型 UDP-糖基转移酶将酪醇转化为特异生产艾卡瑞苷 D2
DOI：
10.1155/2018/7970590
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发表时间：
2018
影响因子：
--
通讯作者：
Chen M
中科院分区：
文献类型：
--
作者：
Liao Z;Qiu F;Zeng J;Gu L;Wang B;Lan X;Chen M
在这项研究中，我们在分子和生化水平上表征了一种新的udp -糖基转移酶（RcUGT1），该酶能将酪醇转化为特异性地从crenuata中产生icariside D2。RcUGT1在花和根中高表达，而icariside D2在茎中的含量远高于其他器官，表明icariside D2可能从花和根转运到茎中。茎中icariside D2的高产量为农民在生产icariside D2时收获茎而不是根提供了合理的建议。重组RcUGT1酶解实验表明，重组RcUGT1能将酪醇特异转化为糖甙D2，其转化值分别为Km 0.97±0.10 mM、Vmax 286±8.26 pKat/mg、Kcat 0.01552 s−1和Kcat/Km 159.55 s−1 M−1。RcUGT1的功能鉴定有助于通过植物代谢工程或微生物合成生物学产生icariside D2。
Rhodiola crenulata is a Tibetan native herbal plant belonging to the family of Crassulaceae, which produces the pharmaceutical icariside D2 with the activities of inhibiting angiotensin-converting enzyme and killing leukemia cancer cells. In this study, we functionally characterized a novel UDP-glycosyltransferase (RcUGT1) that converted tyrosol to specifically produce icariside D2 from R. crenulata at molecular and biochemical levels. RcUGT1 was highly expressed in flowers and roots, while the icariside D2 content was much higher in stems than that in other organs, suggesting the potential translocation of icariside D2 from flowers and roots to stems. The high production of icariside D2 in stems provided a reasonable suggestion to farmers to harvest stems instead of roots for icariside D2 production. Enzymatic assays of recombinant RcUGT1 indicated that it converted tyrosol to specifically form icariside D2, with the values of Km 0.97±0.10 mM, Vmax 286±8.26 pKat/mg, Kcat 0.01552 s−1, and Kcat/Km 159.55 s−1 M−1. Functional identification of RcUGT1 facilitated the icariside D2 production through metabolic engineering in plants or synthetic biology in microbes.
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