Engineering salidroside biosynthetic pathway in hairy root cultures of Rhodiola crenulata based on metabolic characterization of tyrosine decarboxylase.
Engineering salidroside biosynthetic pathway in hairy root cultures of Rhodiola crenulata based on metabolic characterization of tyrosine decarboxylase.
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DOI：
10.1371/journal.pone.0075459
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wang Q
中科院分区：
文献类型：
--
作者：
Lan X;Chang K;Zeng L;Liu X;Qiu F;Zheng W;Quan H;Liao Z;Chen M;Huang W;Liu W;Wang Q
酪氨酸脱羧酶红景天苷生物合成。大花红景天酪氨酸脱羧酶基因（RcTYDC）的代谢特性表明，该基因在红景天苷的生物合成中起重要作用。重组53 kDa RcTYDC将酪氨酸转化为酪胺。SA/MeJA诱导RcTYDC基因表达与红景天苷生物合成的最后一个基因RcUDPGT的表达协同，SA显著上调RcTYDC和RcUDPGT的表达，分别是对照的49倍和36倍。MeJA还显著增加毛状根培养物中RcTYDC和RcUDPGT的表达。RcTYDC和RcUDPGT的组织谱高度相似：茎中的表达水平最高，叶中的表达水平高于花和根。基因表达水平与红景天苷积累水平一致。这表明RcTYDC在红景天苷生物合成中起重要作用。具细齿的。最后，利用RcTYDC基因对红景天苷生物合成途径进行了工程改造。通过代谢工程的策略，过量表达的crenulata毛根。所有转基因株系的RcTYDC表达水平均显著高于非转基因株系。转基因株系的酪胺、酪醇和红景天苷产量分别是非转基因株系的3.21-6.84、1.50-2.19和1.27-3.47倍。总之，RcTYDC过表达促进酪胺生物合成，这促进更多的代谢通量流向下游途径，结果，中间体酪醇积累更多，导致最终产物红景天苷的产量增加。
Tyrosine decarboxylase initializes salidroside biosynthesis. Metabolic characterization of tyrosine decarboxylase gene from Rhodiola crenulata (RcTYDC) revealed that it played an important role in salidroside biosynthesis. Recombinant 53 kDa RcTYDC converted tyrosine into tyramine. RcTYDC gene expression was induced coordinately with the expression of RcUDPGT (the last gene involved in salidroside biosynthesis) in SA/MeJA treatment; the expression of RcTYDC and RcUDPGT was dramatically upregulated by SA, respectively 49 folds and 36 folds compared with control. MeJA also significantly increased the expression of RcTYDC and RcUDPGT in hairy root cultures. The tissue profile of RcTYDC and RcUDPGT was highly similar: highest expression levels found in stems, higher expression levels in leaves than in flowers and roots. The gene expressing levels were consistent with the salidroside accumulation levels. This strongly suggested that RcTYDC played an important role in salidroside biosynthesis in R. crenulata. Finally, RcTYDC was used to engineering salidroside biosynthetic pathway in R. crenulata hairy roots via metabolic engineering strategy of overexpression. All the transgenic lines showed much higher expression levels of RcTYDC than non-transgenic one. The transgenic lines produced tyramine, tyrosol and salidroside at higher levels, which were respectively 3.21–6.84, 1.50–2.19 and 1.27–3.47 folds compared with the corresponding compound in non-transgenic lines. In conclusion, RcTYDC overexpression promoted tyramine biosynthesis that facilitated more metabolic flux flowing toward the downstream pathway and as a result, the intermediate tyrosol was accumulated more that led to the increased production of the end-product salidroside.
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影响因子：
3
作者：
Zhao, Yan;Sun, Wei;Liu, Chun-Zhao
通讯作者：
Liu, Chun-Zhao
DOI：
10.1073/pnas.89.24.11799
发表时间：
1992-12-15
影响因子：
11.1
作者：
YUN, DJ;HASHIMOTO, T;YAMADA, Y
通讯作者：
YAMADA, Y
影响因子：
5.4
作者：
Bartley, Glenn E.;Breksa, Andrew P., III;Ishida, Betty K.
通讯作者：
Ishida, Betty K.
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.
影响因子：
2
作者：
Zhou, Xiaofu;Wu, Yuxia;Xu, Hongwei
通讯作者：
Xu, Hongwei