Production of chitooligosaccharides and their potential applications in medicine.
Production of chitooligosaccharides and their potential applications in medicine.
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DOI：
10.3390/md8051482
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发表时间：
2010-04-27
期刊：
影响因子：
5.4
通讯作者：
Eijsink VG
中科院分区：
文献类型：
--
作者：
Aam BB;Heggset EB;Norberg AL;Sørlie M;Vårum KM;Eijsink VG
壳寡糖（CHOS）是N-乙酰葡糖胺和D-葡糖胺的同源或异源寡聚物。可以使用几丁质或壳聚糖作为起始材料，使用酶促转化、化学方法或其组合来生产CHOS。由于这些寡糖被认为具有几种有趣的生物活性，因此生产定义明确的CHOS混合物或甚至纯CHOS具有极大的意义。了解这些生物活性的机制是非常重要的。然而，到目前为止，深入了解的模式的行动的CHOS是稀缺的，一个主要的原因是，大多数已发表的研究是做了严重的特点异质混合物的CHOS。在长度、N-乙酰化程度和序列方面明确定义的CHOS的产生并不简单。在这里，我们提供了一个概述的技术，可用于生产和表征合理定义的CHOS馏分。我们还提出了CHOS可能的医学应用，包括肿瘤生长抑制和抑制TH 2诱导的哮喘炎症，以及用作骨质疏松症的骨强化剂，基因递送载体，抗菌剂，抗真菌剂，抗疟疾剂或伤口敷料中的止血剂。通过使用明确定义的CHOS混合物，将有可能更好地了解这些生物活性的机制。
Chitooligosaccharides (CHOS) are homo- or heterooligomers of N-acetylglucosamine and D-glucosamine. CHOS can be produced using chitin or chitosan as a starting material, using enzymatic conversions, chemical methods or combinations thereof. Production of well-defined CHOS-mixtures, or even pure CHOS, is of great interest since these oligosaccharides are thought to have several interesting bioactivities. Understanding the mechanisms underlying these bioactivities is of major importance. However, so far in-depth knowledge on the mode-of-action of CHOS is scarce, one major reason being that most published studies are done with badly characterized heterogeneous mixtures of CHOS. Production of CHOS that are well-defined in terms of length, degree of N-acetylation, and sequence is not straightforward. Here we provide an overview of techniques that may be used to produce and characterize reasonably well-defined CHOS fractions. We also present possible medical applications of CHOS, including tumor growth inhibition and inhibition of TH2-induced inflammation in asthma, as well as use as a bone-strengthener in osteoporosis, a vector for gene delivery, an antibacterial agent, an antifungal agent, an anti-malaria agent, or a hemostatic agent in wound-dressings. By using well-defined CHOS-mixtures it will become possible to obtain a better understanding of the mechanisms underlying these bioactivities.
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影响因子：
11.2
作者：
Cederkvist, F. Henning;Parmer, Marthe P.;Sorlie, Morten
通讯作者：
Sorlie, Morten
影响因子：
158.5
作者：
Chupp, Geoffrey L.;Lee, Chun Geun;Elias, Jack A.
通讯作者：
Elias, Jack A.
DOI：
10.1016/s0006-291x(03)00173-6
发表时间：
2003-03-14
影响因子：
3.1
作者：
Chou, TC;Fu, E;Yeh, JH
通讯作者：
Yeh, JH
影响因子：
3.1
作者：
Dessens, JT;Mendoza, J;Sinden, RE
通讯作者：
Sinden, RE
影响因子：
5.7
作者：
DAVIES, G;HENRISSAT, B
通讯作者：
HENRISSAT, B