Exploiting algal NADPH oxidase for biophotovoltaic energy.
Exploiting algal NADPH oxidase for biophotovoltaic energy.
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DOI：
10.1111/pbi.12332
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发表时间：
2016-01
影响因子：
13.8
通讯作者：
Smith AG
中科院分区：
文献类型：
--
作者：
Anderson A;Laohavisit A;Blaby IK;Bombelli P;Howe CJ;Merchant SS;Davies JM;Smith AG
光合微生物表现出光依赖性的电子输出穿过细胞膜，这可以在生物光伏（BPV）装置中发电。电子如何输出仍有待确定;机制的识别将有助于选择或产生能够增强电输出的光合微生物。我们表明，质膜NADPH氧化酶活性是光依赖性发电的单细胞绿色衣藻莱茵衣藻的重要组成部分。NADPH氧化酶输出电子穿过质膜以从氧形成超氧阴离子。缺乏RBO 1编码的NADPH氧化酶的莱茵衣藻突变体在BPV装置中的细胞外超氧阴离子产生和电流产生方面都受到损害。与野生型基因的互补恢复了这两种能力，证明了酶在电子输出中的作用。用比色测定法监测光依赖性胞外超氧化物的产生被证明是筛选候选藻株的产电潜力的有效方式。结果表明，藻类NADPH氧化酶是重要的超氧阴离子的生产和开放的途径，优化这些设备的生物组成。
Photosynthetic microbes exhibit light‐dependent electron export across the cell membrane, which can generate electricity in biological photovoltaic (BPV) devices. How electrons are exported remains to be determined; the identification of mechanisms would help selection or generation of photosynthetic microbes capable of enhanced electrical output. We show that plasma membrane NADPH oxidase activity is a significant component of light‐dependent generation of electricity by the unicellular green alga Chlamydomonas reinhardtii. NADPH oxidases export electrons across the plasma membrane to form superoxide anion from oxygen. The C. reinhardtii mutant lacking the NADPH oxidase encoded by RBO1 is impaired in both extracellular superoxide anion production and current generation in a BPV device. Complementation with the wild‐type gene restores both capacities, demonstrating the role of the enzyme in electron export. Monitoring light‐dependent extracellular superoxide production with a colorimetric assay is shown to be an effective way of screening for electrogenic potential of candidate algal strains. The results show that algal NADPH oxidases are important for superoxide anion production and open avenues for optimizing the biological component of these devices.
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