Comparative genomics of multiple strains of Pseudomonas cannabina pv. alisalensis, a potential model pathogen of both monocots and dicots.
Comparative genomics of multiple strains of Pseudomonas cannabina pv. alisalensis, a potential model pathogen of both monocots and dicots.
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DOI：
10.1371/journal.pone.0059366
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Goumas DE
中科院分区：
文献类型：
--
作者：
Sarris PF;Trantas EA;Baltrus DA;Bull CT;Wechter WP;Yan S;Ververidis F;Almeida NF;Jones CD;Dangl JL;Panopoulos NJ;Vinatzer BA;Goumas DE
对宿主范围不同的密切相关病原体进行比较基因组学研究，可以深入了解宿主-病原体相互作用和宿主适应的机制。此外，具有相同宿主范围的多个菌株的测序揭示了有关病原体多样性和毒力的分子基础的信息。本文对四株大麻假单胞菌（Pseudomonas cannabina pathovar alisalensis, Pcal）的基因组草图序列进行了比较分析，该菌株对一系列单子叶和双子叶植物具有致病性。这些草图的基因组序列为理解寄主范围的进化提供了基础。与其他植物致病性假单胞菌一样，Pcal菌株含有hrp/hrc基因簇，该基因簇编码III型分泌系统。基于hrp/hrc集群基因/蛋白的系统发育分析表明，hrp/hrc集群内部存在局部重组和功能分化。尽管整个Pcal基因组的总体遗传含量显著保守，但III型效应基因库的比较强化了先前的分子数据，表明该疾病中存在两个不同的谱系。此外，所有被分析的Pcal菌株都有两个不同的基因组岛，预测它们编码VI型分泌系统（t6ss）。虽然其中一个系统与已知的紫丁香假单胞菌T6SS同源，但另一个系统更接近铜绿假单胞菌中的T6SS。总之，我们的研究为揭示单子叶和双子叶寄主对Pcal的适应提供了基础，并为潜在的致病机制提供了遗传学见解。
Comparative genomics of closely related pathogens that differ in host range can provide insights into mechanisms of host-pathogen interactions and host adaptation. Furthermore, sequencing of multiple strains with the same host range reveals information concerning pathogen diversity and the molecular basis of virulence. Here we present a comparative analysis of draft genome sequences for four strains of Pseudomonas cannabina pathovar alisalensis (Pcal), which is pathogenic on a range of monocotyledonous and dicotyledonous plants. These draft genome sequences provide a foundation for understanding host range evolution across the monocot-dicot divide. Like other phytopathogenic pseudomonads, Pcal strains harboured a hrp/hrc gene cluster that codes for a type III secretion system. Phylogenetic analysis based on the hrp/hrc cluster genes/proteins, suggests localized recombination and functional divergence within the hrp/hrc cluster. Despite significant conservation of overall genetic content across Pcal genomes, comparison of type III effector repertoires reinforced previous molecular data suggesting the existence of two distinct lineages within this pathovar. Furthermore, all Pcal strains analyzed harbored two distinct genomic islands predicted to code for type VI secretion systems (T6SSs). While one of these systems was orthologous to known P. syringae T6SSs, the other more closely resembled a T6SS found within P. aeruginosa. In summary, our study provides a foundation to unravel Pcal adaptation to both monocot and dicot hosts and provides genetic insights into the mechanisms underlying pathogenicity.
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影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
影响因子：
4.5
作者：
Cintas, NA;Koike, ST;Bull, CT
通讯作者：
Bull, CT
影响因子：
6.7
作者：
Baltrus DA;Nishimura MT;Romanchuk A;Chang JH;Mukhtar MS;Cherkis K;Roach J;Grant SR;Jones CD;Dangl JL
通讯作者：
Dangl JL
影响因子：
4.5
作者：
Abby SS;Rocha EP
通讯作者：
Rocha EP
DOI：
10.1073/pnas.1731982100
发表时间：
2003-09-02
影响因子：
11.1
作者：
Buell, CR;Joardar, V;Collmer, A
通讯作者：
Collmer, A