Comparative metagenomics of anode-associated microbiomes developed in rice paddy-field microbial fuel cells.
Comparative metagenomics of anode-associated microbiomes developed in rice paddy-field microbial fuel cells.
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DOI：
10.1371/journal.pone.0077443
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Watanabe K
中科院分区：
文献类型：
--
作者：
Kouzuma A;Kasai T;Nakagawa G;Yamamuro A;Abe T;Watanabe K
在稻田沉积型微生物燃料电池（sMFC）中，水稻根际微生物将水稻根分泌物转化为电能。以往的研究表明，根际sMFC的阳极上富集了土杆菌科的成员。为了加深我们对sMFC中参与发电的根际微生物的理解，在这里，我们对三种MFC系统中的阳极相关微生物组进行了比较分析：稻田sMFC，以及乙酸盐和葡萄糖喂养的MFC，其中在稻田sMFC中用作阳极的石墨毡片被用作根际微生物承载阳极。在电输出变得稳定后，通过16S rRNA基因扩增子的pyrotag测序和Illumina鸟枪宏基因组学分析与这些MFC系统的阳极相关的微生物组。Pyrotag测序结果表明，Geobacteraceae细菌与所有三个系统的阳极，但在每个MFC的优势Geobacteraceae物种是不同的。特别是与G.金属还原菌在乙酸盐源MFC中占阳极土杆菌科的90%，但在根际sMFC和葡萄糖源MFC中仅占相对较少的组分，而与G.大量检测到嗜冷菌。阳极微生物组鸟枪法宏基因组序列中预测基因的系统发育分配证实了这一趋势。我们的研究结果表明G.嗜冷菌及其相关物种优先生长在根际sMFC的阳极上，并通过与分泌电子供体的生物体的互养相互作用来发电。
In sediment-type microbial fuel cells (sMFCs) operating in rice paddy fields, rice-root exudates are converted to electricity by anode-associated rhizosphere microbes. Previous studies have shown that members of the family Geobacteraceae are enriched on the anodes of rhizosphere sMFCs. To deepen our understanding of rhizosphere microbes involved in electricity generation in sMFCs, here, we conducted comparative analyses of anode-associated microbiomes in three MFC systems: a rice paddy-field sMFC, and acetate- and glucose-fed MFCs in which pieces of graphite felt that had functioned as anodes in rice paddy-field sMFC were used as rhizosphere microbe-bearing anodes. After electric outputs became stable, microbiomes associated with the anodes of these MFC systems were analyzed by pyrotag sequencing of 16S rRNA gene amplicons and Illumina shotgun metagenomics. Pyrotag sequencing showed that Geobacteraceae bacteria were associated with the anodes of all three systems, but the dominant Geobacter species in each MFC were different. Specifically, species closely related to G. metallireducens comprised 90% of the anode Geobacteraceae in the acetate-fed MFC, but were only relatively minor components of the rhizosphere sMFC and glucose-fed MFC, whereas species closely related to G. psychrophilus were abundantly detected. This trend was confirmed by the phylogenetic assignments of predicted genes in shotgun metagenome sequences of the anode microbiomes. Our findings suggest that G. psychrophilus and its related species preferentially grow on the anodes of rhizosphere sMFCs and generate electricity through syntrophic interactions with organisms that excrete electron donors.
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影响因子：
14.9
作者：
Kanehisa M;Goto S;Sato Y;Furumichi M;Tanabe M
通讯作者：
Tanabe M
影响因子：
11.4
作者：
de Schamphelaire, Liesje;van den Bossche, Leen;Verstraete, Willy
通讯作者：
Verstraete, Willy
影响因子：
11.4
作者：
Freguia, Stefano;Rabaey, Korneel;Keller, Juerg
通讯作者：
Keller, Juerg
影响因子：
5
作者：
Jung, Sokhee;Regan, John M.
通讯作者：
Regan, John M.
DOI：
10.1099/00207713-44-4-812
发表时间：
1994-10-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
COLLINS, MD;LAWSON, PA;FARROW, JAE
通讯作者：
FARROW, JAE