Characterization of oral swab samples for diagnosis of pulmonary tuberculosis.
Characterization of oral swab samples for diagnosis of pulmonary tuberculosis.
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口服拭子样品的表征用于诊断肺结核。
DOI：
10.1371/journal.pone.0251422
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Minch KJ
中科院分区：
文献类型：
--
作者：
Wood RC;Andama A;Hermansky G;Burkot S;Asege L;Job M;Katumba D;Nakaye M;Mwebe SZ;Mulondo J;Bachman CM;Nichols KP;Le Ny AM;Ortega C;Olson RN;Weigel KM;Olson AM;Madan D;Bell D;Cattamanchi A;Worodria W;Semitala FC;Somoskovi A;Cangelosi GA;Minch KJ
口腔拭子分析(OSA)已被证明可以检测肺结核(TB)患者中的结核分枝杆菌(MTB)DNA。在以前的分析中，从舌背采集的拭子样本的qPCR检测相对于痰GeneXpert的敏感性高达93%，当每个患者检测2个拭子时。本研究改进了样本采集方法，以增加样本生物量，并表征了舌拭子中存在的杆菌的活性。针对保守的细菌核糖体rRNA基因(RDNA)序列的qPCR被用来定量样品中的细菌生物量。在10次快速重复的舌样过程中，细菌总rDNA信号没有明显减少，这表明拭子只收集了可供检测的生物量的一小部分。Copan FLOQSwabs收集的生物量比以前使用的最好的品牌Purflock棉签多2倍(p=0.006)。因此，在乌干达可能患有结核病的患者中对FLOQSwabs进行了评估。在登记时(第1天)从每个患者身上收集FLOQSwb，在GeneXpert超阳性患者的一个子集中，在第二天(第2天)收集第二个拭子。用手动IS6110靶向定量聚合酶链式反应检测拭子中的结核杆菌DNA。与GeneXpert Ultra相比，单拭子检测第1天的敏感性为88%(44/50)，第2天的敏感性为94.4%(17/18)，特异性为79.2%(42/53)。在扩大的乌干达患者样本中，62%(87/141)的舌背拭子样本中有菌落形成杆菌。这些发现将有助于指导这一前景光明的结核病筛查方法的进一步发展。
Oral swab analysis (OSA) has been shown to detect Mycobacterium tuberculosis (MTB) DNA in patients with pulmonary tuberculosis (TB). In previous analyses, qPCR testing of swab samples collected from tongue dorsa was up to 93% sensitive relative to sputum GeneXpert, when 2 swabs per patient were tested. The present study modified sample collection methods to increase sample biomass and characterized the viability of bacilli present in tongue swabs. A qPCR targeting conserved bacterial ribosomal rRNA gene (rDNA) sequences was used to quantify bacterial biomass in samples. There was no detectable reduction in total bacterial rDNA signal over the course of 10 rapidly repeated tongue samplings, indicating that swabs collect only a small portion of the biomass available for testing. Copan FLOQSwabs collected ~2-fold more biomass than Puritan PurFlock swabs, the best brand used previously (p = 0.006). FLOQSwabs were therefore evaluated in patients with possible TB in Uganda. A FLOQSwab was collected from each patient upon enrollment (Day 1) and, in a subset of sputum GeneXpert Ultra-positive patients, a second swab was collected on the following day (Day 2). Swabs were tested for MTB DNA by manual IS6110-targeted qPCR. Relative to sputum GeneXpert Ultra, single-swab sensitivity was 88% (44/50) on Day 1 and 94.4% (17/18) on Day 2. Specificity was 79.2% (42/53). Among an expanded sample of Ugandan patients, 62% (87/141) had colony-forming bacilli in their tongue dorsum swab samples. These findings will help guide further development of this promising TB screening method.
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影响因子：
9.4
作者：
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通讯作者：
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影响因子：
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DOI：
10.1016/j.diagmicrobio.2019.114892
发表时间：
2020-01-01
影响因子：
2.9
作者：
Andama, A.;Jaganath, D.;Cattamanchi, A.
通讯作者：
Cattamanchi, A.
影响因子：
3.9
作者：
Molina-Moya B;Ciobanu N;Hernandez M;Prat-Aymerich C;Crudu V;Adams ER;Codreanu A;Sloan DJ;Cuevas LE;Dominguez J
通讯作者：
Dominguez J