Protein cleavage influences surface protein presentation in Mycoplasma pneumoniae.
Protein cleavage influences surface protein presentation in Mycoplasma pneumoniae.
复制标题
蛋白裂解影响肺炎支原体表面蛋白的表达。
DOI：
10.1038/s41598-021-86217-y
复制
发表时间：
2021-03-24
影响因子：
4.6
通讯作者：
Djordjevic SP
中科院分区：
文献类型：
--
作者：
Berry IJ;Widjaja M;Jarocki VM;Steele JR;Padula MP;Djordjevic SP
肺炎支原体是肺炎的一个重要原因，感染后后遗症影响器官部位远离呼吸道是常见的。它也是一种模式生物，在那里进行了广泛的“组学”研究，以深入了解最小基因组自我复制生物体的功能。在此进行的N-末端组研究鉴定了4898个独特的N-末端肽，映射到391个（56%）预测的M。pneumoniae蛋白从预测的开放阅读框架（ORF）的起始甲硫氨酸（iMet）残基开始的真正的N-末端序列被确定为163种蛋白质。值得注意的是，几乎一半（317; 46%）的ORFS来自M。pneumoniae菌株M129是后修饰的，推测是通过蛋白水解加工，因为二甲基标记的新-N-末端被表征为定位在预测的N-末端之外。对N-末端组的分析描述了主要靶向胰蛋白酶样位点的内切蛋白水解加工事件，尽管P1′（D和E）中带负电荷的残基与P2′和P3′位置的赖氨酸或丝氨酸/丙氨酸的裂解也经常发生。表面组研究鉴定了160种蛋白质（占蛋白质组的23%）暴露在M的细胞外表面。肺炎。两种正交方法用于鉴定表面组，每种方法鉴定了相同的116种蛋白质，重叠率为72%（116/160）。除了脂蛋白、转运蛋白和粘附素外，93/160（58%）的表面蛋白缺乏信号肽，并在细胞中具有良好的典型功能。在鉴定的160种表面蛋白中，134种也是内切蛋白水解加工的靶标。这些处理事件可能对宿主免疫系统如何识别和响应M.肺炎。
Mycoplasma pneumoniae is a significant cause of pneumonia and post infection sequelae affecting organ sites distant to the respiratory tract are common. It is also a model organism where extensive ‘omics’ studies have been conducted to gain insight into how minimal genome self-replicating organisms function. An N-terminome study undertaken here identified 4898 unique N-terminal peptides that mapped to 391 (56%) predicted M. pneumoniae proteins. True N-terminal sequences beginning with the initiating methionine (iMet) residue from the predicted Open Reading Frame (ORF) were identified for 163 proteins. Notably, almost half (317; 46%) of the ORFS derived from M. pneumoniae strain M129 are post-translationally modified, presumably by proteolytic processing, because dimethyl labelled neo-N-termini were characterised that mapped beyond the predicted N-terminus. An analysis of the N-terminome describes endoproteolytic processing events predominately targeting tryptic-like sites, though cleavages at negatively charged residues in P1′ (D and E) with lysine or serine/alanine in P2′ and P3′ positions also occurred frequently. Surfaceome studies identified 160 proteins (23% of the proteome) to be exposed on the extracellular surface of M. pneumoniae. The two orthogonal methodologies used to characterise the surfaceome each identified the same 116 proteins, a 72% (116/160) overlap. Apart from lipoproteins, transporters, and adhesins, 93/160 (58%) of the surface proteins lack signal peptides and have well characterised, canonical functions in the cell. Of the 160 surface proteins identified, 134 were also targets of endo-proteolytic processing. These processing events are likely to have profound implications for how the host immune system recognises and responds to M. pneumoniae.
登录
查看更多内容
影响因子：
6.4
作者：
Kawamoto A;Matsuo L;Kato T;Yamamoto H;Namba K;Miyata M
通讯作者：
Miyata M
细胞内蛋白质的物理特征，在细胞表面上月光。
影响因子：
3.7
作者：
Amblee V;Jeffery CJ
通讯作者：
Jeffery CJ
影响因子：
3.6
作者：
Burnett, TA;Dinkla, K;Djordjevic, SP
通讯作者：
Djordjevic, SP
影响因子：
4.2
作者：
Bendtsen JD;Kiemer L;Fausbøll A;Brunak S
通讯作者：
Brunak S
影响因子：
14.9
作者：
Himmelreich, R;Hilbert, H;Herrmann, R
通讯作者：
Herrmann, R