Construction of a genetic map for Citrullus lanatus based on CAPS markers and mapping of three qualitative traits

Construction of a genetic map for Citrullus lanatus based on CAPS markers and mapping of three qualitative traits
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基于CAPS标记的西瓜遗传图谱构建及三个性状定位

DOI:
10.1016/j.scienta.2017.10.029
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发表时间:
2018-03
影响因子:
4.3
通讯作者:
王学征
王学征
中科院分区:
农林科学2区
文献类型:
--
作者:
张志鹏;张雅楠;王学征

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以浅绿色皮、红色果肉、无苦味栽培西瓜自交系W1-1与深绿色皮、白色肉、苦味野生种PI 186490及其母本回交F1代,获得225个BC 1代。基于全基因组重测序数据,使用跨越全基因组的CAPS标记对每个个体进行基因分型。利用186个CAPS标记构建了西瓜遗传连锁图谱,总距离为1468.09cM。以肉色、果皮花纹和肉苦味为3个品质性状进行遗传分析和基因定位。结果表明,果皮宽条纹和果肉苦味受1对显性基因控制,红色果肉对白色果肉为隐性基因,共检测到3个与上述3个性状相关的基因座。本研究为西瓜果实性状的分子标记辅助选择提供了理论依据。
Two-hundred-twenty-five BC1generations were obtained from backcrossing F1of the shallow green rind, red flesh, no bitterness cultivated watermelon inbred line W1-1 and the dark green rind, white flesh, bitter wild species PI186490 hybrid as well as their female parents. CAPS makers spanning the whole genome were used to genotype every individual based on whole-genome resequencing data. Watermelon genetic linkage maps of 186 CAPS markers corresponding to 11 chromosomes were constructed, spanning a total distance of 1468.09 cM. Genetic analysis and gene mapping were performed using the flesh color, rind pattern and flesh bitterness as 3 qualitative traits. The results showed that wide rind stripes and flesh bitterness were controlled by a dominant gene, with red flesh recessive to white flesh, and 3 loci were identified related to the 3 fruit traits mentioned above. This study provides a theoretical basis for molecular marker-assisted selection of desirable watermelon fruit traits.
基于四个作图群体和与西瓜(Citrullus lanatus)重要经济性状相关的数量性状位点的综合遗传图谱
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