Metabolic disease risk in children by salivary biomarker analysis.
Metabolic disease risk in children by salivary biomarker analysis.
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DOI：
10.1371/journal.pone.0098799
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Welty F
中科院分区：
文献类型：
--
作者：
Goodson JM;Kantarci A;Hartman ML;Denis GV;Stephens D;Hasturk H;Yaskell T;Vargas J;Wang X;Cugini M;Barake R;Alsmadi O;Al-Mutawa S;Ariga J;Soparkar P;Behbehani J;Behbehani K;Welty F
由于害怕针头，儿童肥胖相关代谢综合征或2型糖尿病（T2 D）的研究特别困难。我们测试了一种非侵入性的方法来研究高危儿童人群的炎症参数，为将来对脆弱受试者的研究提供原理证明。我们通过分析空腹唾液样本中的20种生物标志物，评估了744名11岁儿童的代谢差异，这些儿童选自体重不足、正常健康体重、超重和肥胖类别。离心后获得唾液上清液并用于分析。肥胖儿童的唾液C-反应蛋白（CRP）是正常体重儿童的6倍，唾液胰岛素和瘦素是正常体重儿童的3倍，脂联素是正常体重儿童的30%（均P<0.0001）。分类分析表明，儿童肥胖可能有三种类型。76%的肥胖儿童出现明显的炎症特征，而13%的肥胖儿童唾液胰岛素高，但与炎症介质无关。其余11%的肥胖儿童胰岛素水平高，脂联素水平低。40%的非肥胖儿童是在基于生物标志物特征的可能有肥胖风险的群体中发现的。来自高危人群的肥胖儿童唾液生物标志物水平的显著改变表明，有可能开发非侵入性筛查程序来识别T2 D易感个体，并测试预防策略。
The study of obesity-related metabolic syndrome or Type 2 diabetes (T2D) in children is particularly difficult because of fear of needles. We tested a non-invasive approach to study inflammatory parameters in an at-risk population of children to provide proof-of-principle for future investigations of vulnerable subjects. We evaluated metabolic differences in 744, 11-year old children selected from underweight, normal healthy weight, overweight and obese categories by analyzing fasting saliva samples for 20 biomarkers. Saliva supernatants were obtained following centrifugation and used for analyses. Salivary C-reactive protein (CRP) was 6 times higher, salivary insulin and leptin were 3 times higher, and adiponectin was 30% lower in obese children compared to healthy normal weight children (all P<0.0001). Categorical analysis suggested that there might be three types of obesity in children. Distinctly inflammatory characteristics appeared in 76% of obese children while in 13%, salivary insulin was high but not associated with inflammatory mediators. The remaining 11% of obese children had high insulin and reduced adiponectin. Forty percent of the non-obese children were found in groups which, based on biomarker characteristics, may be at risk for becoming obese. Significantly altered levels of salivary biomarkers in obese children from a high-risk population, suggest the potential for developing non-invasive screening procedures to identify T2D-vulnerable individuals and a means to test preventative strategies.
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DOI：
10.4049/jimmunol.1002615
发表时间：
2011-01-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Jagannathan-Bogdan M;McDonnell ME;Shin H;Rehman Q;Hasturk H;Apovian CM;Nikolajczyk BS
通讯作者：
Nikolajczyk BS
影响因子：
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作者：
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通讯作者：
Malamud D
影响因子：
5.8
作者：
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通讯作者：
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DOI：
10.1196/annals.1384.028
发表时间：
2007-01-01
期刊：
ORAL-BASED DIAGNOSTICS
影响因子：
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作者：
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通讯作者：
Giannobile, William V.
DOI：
10.1073/pnas.1215840110
发表时间：
2013-03-26
影响因子：
11.1
作者：
DeFuria, Jason;Belkina, Anna C.;Nikolajczyk, Barbara S.
通讯作者：
Nikolajczyk, Barbara S.