The gravity-induced re-localization of auxin efflux carrier CsPIN1 in cucumber seedlings: spaceflight experiments for immunohistochemical microscopy.
The gravity-induced re-localization of auxin efflux carrier CsPIN1 in cucumber seedlings: spaceflight experiments for immunohistochemical microscopy.
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DOI：
10.1038/npjmgrav.2016.30
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发表时间：
2016
期刊：
影响因子：
5.1
通讯作者：
Takahashi H
中科院分区：
文献类型：
--
作者：
Yamazaki C;Fujii N;Miyazawa Y;Kamada M;Kasahara H;Osada I;Shimazu T;Fusejima Y;Higashibata A;Yamazaki T;Ishioka N;Takahashi H
黄瓜幼苗的重新定位诱导CsPIN1生长素外排载体在下胚轴和根之间的过渡区的内胚层细胞中的重新定位。本研究探讨了CsPIN 1的重新定位是否是由于重力反应。免疫组织化学分析表明，当黄瓜幼苗在太空微重力条件下完全生长时，内胚层细胞中的CsPIN 1主要定位于平行于过渡区椭圆形横截面短轴的细胞侧。然而，当黄瓜种子在微重力下萌发24 h，然后暴露于空间中的方向垂直于幼苗轴的1g离心2 h，CsPIN 1重新定位于过渡区的内胚层细胞的底部。这些结果表明，CsPIN 1的定位在内胚层细胞的变化响应重力。此外，我们的研究结果表明，内胚层细胞层成为一个渠道，生长素是横向运输从上到下侧翼在重力的反应。CsPIN 1的重力反应调节的重新定位可能是负责生长素水平的下降，从而抑制PEG的形成，在上方的过渡区水平放置的黄瓜幼苗。
Reorientation of cucumber seedlings induces re-localization of CsPIN1 auxin efflux carriers in endodermal cells of the transition zone between hypocotyl and roots. This study examined whether the re-localization of CsPIN1 was due to the graviresponse. Immunohistochemical analysis indicated that, when cucumber seedlings were grown entirely under microgravity conditions in space, CsPIN1 in endodermal cells was mainly localized to the cell side parallel to the minor axis of the elliptic cross-section of the transition zone. However, when cucumber seeds were germinated in microgravity for 24 h and then exposed to 1g centrifugation in a direction crosswise to the seedling axis for 2 h in space, CsPIN1 was re-localized to the bottom of endodermal cells of the transition zone. These results reveal that the localization of CsPIN1 in endodermal cells changes in response to gravity. Furthermore, our results suggest that the endodermal cell layer becomes a canal by which auxin is laterally transported from the upper to the lower flank in response to gravity. The graviresponse-regulated re-localization of CsPIN1 could be responsible for the decrease in auxin level, and thus for the suppression of peg formation, on the upper side of the transition zone in horizontally placed seedlings of cucumber.
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影响因子：
2.7
作者：
Hellsberg E;Montanari F;Ecker GF
通讯作者：
Ecker GF
影响因子：
2.8
作者：
Shimamura, Masaki;Yamaguchi, Tomio;Deguchi, Hironori
通讯作者：
Deguchi, Hironori
影响因子：
4.3
作者：
Kraft, TFB;van Loon, JJWA;Kiss, JZ
通讯作者：
Kiss, JZ
影响因子：
4.3
作者：
Kamada, M;Yamasaki, S;Takahashi, H
通讯作者：
Takahashi, H
影响因子：
--
作者：
Went, FAFC
通讯作者：
Went, FAFC